Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

07 August 2002

Three-dimensional structure and molecular mechanism of novel enzymes of spore-forming bacteria

Mark J. Jedrzejas

Med Sci Monit 2002; 8(8): RA183-190 :: ID: 13610

Abstract

Summary
Bacillus and Clostridium species are spore-forming bacilli that cause serious diseases in livestock and in humans. An important pathogen in this group of organisms is Bacillus anthracis is which is the causative agent of anthrax. The biochemical properties, structure, function, and mechanism of catalysis of the novel spore germination protease (GPR) that degrades small, acid soluble proteins (SASP) protecting spore’s DNA against damage and a novel, cofactor independent
phosphoglycerate mutase (iPGM) are described. A coat created from SASP synthesized during sporulation protects DNA in spores and later in the germination this protective coat is removal by a novel GPR protease. GPR does not resemble in its structure any other known
protein and functionally likely belongs to a novel class of proteases utilizing glutamic acid residue in its catalysis in order to degrade SASP. This process provides amino acids necessary for synthesis of other proteins or enzymes necessary in vegetative state. In addition during the spore creation process energy is stored in the form of 3-phosphoglycerate by downregulating a novel, diphosphoglycerate independent iPGM. These depots of energy are utilized later by
the germinating spore in addition to the above source of amino acids. The two above enzymes are inherently involved in the processes of sporulation, spore germination and outgrowth. A combination of structural investigations and site-directed mutagenesis studies that were used to characterize the functional properties and the active sites of these novel proteins will be discussed. The understanding of functional properties of these enzymes contributes to a better
understanding of spore-forming organisms.

Keywords: Bacillus - enzymology, Bacillus - physiology, Bacterial Proteins - metabolism, Binding Sites, Clostridium - enzymology, DNA, Bacterial - metabolism, DNA-Binding Proteins - metabolism, Endopeptidases - chemistry, Endopeptidases - metabolism, Models, Molecular, Phosphoglycerate Mutase - chemistry, Phosphoglycerate Mutase - metabolism, Protein Conformation, Spores, Bacterial - enzymology

Add Comment 0 Comments

Editorial

01 November 2024 : Editorial  

Editorial: Artificial Intelligence (AI), Digital Image Analysis, and the Future of Cancer Diagnosis and Prognosis

Dinah V. Parums

DOI: 10.12659/MSM.947038

Med Sci Monit 2024; 30:e947038

0:00

In Press

Database Analysis  

Impact of Tigecycline on Coagulation in Severe Infections and Effect of Vitamin K1 Intervention: A Retrospe...

Med Sci Monit In Press; DOI: 10.12659/MSM.944778  

Animal Research  

Impact of Fresh Khat Extract on Proinflammatory Cytokines and Hepatic and Renal Responses

Med Sci Monit In Press; DOI: 10.12659/MSM.946108  

Review article  

Epigenetic and Immune Mechanisms Linking Breastfeeding to Lower Breast Cancer Rates

Med Sci Monit In Press; DOI: 10.12659/MSM.945451  

Clinical Research  

Comprehensive Analysis of Immune Infiltration and Key Genes in Peri-Implantitis Using Bioinformatics and Mo...

Med Sci Monit In Press; DOI: 10.12659/MSM.941870  

Most Viewed Current Articles

17 Jan 2024 : Review article   6,085,453

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research   1,907,170

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research   695,059

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

07 Jan 2022 : Meta-Analysis   259,783

Efficacy and Safety of Light Therapy as a Home Treatment for Motor and Non-Motor Symptoms of Parkinson Dise...

DOI :10.12659/MSM.935074

Med Sci Monit 2022; 28:e935074

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750