Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 January 2005

Glutamate Uptake by Rat Brain Astroglia Incubated in Human Cerebrospinal Fluid

Jorge A Colombo

Med Sci Monit 2005; 11(1): BR13-17 :: ID: 13865

Abstract

Background: The glial compartment is critical for the regulation of glutamate concentration within the extracellular space. Reportedly, several factors infl uence glutamate uptake and metabolism in rat astroglia, which also appears to be affected by regional variables. In vitro, glutamate uptake by astroglia grown and tested in artifi cial media is affected by extracellular potassium. This report analyzes tamate uptake by astroglia tested in a physiological extracelullar medium, cerebrospinal fl uid (CSF), instead of artifi cial media.Material/Methods: Astroglial enriched subcultures of fronto-parietal cerebral cortex and striatum from 5-day-old rat pups were used to study glutamate uptake, using human CSF as the extracellular medium. Results: Cultured astrocytes display signifi cant glutamate uptake. This uptake is increased if the potassium concentration is elevated from 3.45 to 12.0 mM in culture medium, but not in the presence of CSF. Differential effects of the glial inhibitor L-a-aminoadipic acid (L-a-AA) were observed at high concentrations in the presence of CSF, compared with standard incubation conditions. This was not observed when D-aspartate was used as a specifi c uptake competitor, suggesting that changes mediated by the presence of CSF components may not be directly linked to the primary transport mechanism, but could interfere at a more non-specifi c level.
Conclusions: These results confi rm data from previous work on regional astroglial differences in glutamatergic management. Yet increased glutamate uptake following changes in extracellular potassium concentration depended on the nature of the culture medium used, since replacement of artifi cial culture medium by CSF resulted in no such increase.

Keywords: Astrocytes - metabolism, Cerebral Cortex - cytology, Cerebrospinal Fluid - metabolism, Corpus Striatum - cytology, Culture Media, Extracellular Fluid - metabolism, Glutamic Acid - metabolism, Potassium - metabolism, Astrocytes - metabolism, Cerebral Cortex - cytology, Cerebrospinal Fluid - metabolism, Corpus Striatum - cytology, Culture Media, Extracellular Fluid - metabolism, Glutamic Acid - metabolism, Potassium - metabolism

Add Comment 0 Comments

Editorial

01 February 2024 : Editorial  

Editorial: Global Health Concerns as Vaccine-Preventable Infections Including SARS-CoV-2 (JN.1), Influenza, Respiratory Syncytial Virus (RSV), and Measles Continue to Rise

Dinah V. Parums

DOI: 10.12659/MSM.943911

Med Sci Monit 2024; 30:e943911

0:00

In Press

23 Feb 2024 : Clinical Research  

Early Mobilization in Pediatric Critical Care: Exploring the Gap Between Theory and Practice in Saudi Arabia

Med Sci Monit In Press; DOI: 10.12659/MSM.942467  

0:00

01 Feb 2024 : Review article  

Enhancing Patient Adherence to Newly-Prescribed Medicine for Chronic Diseases: A Comprehensive Review and C...

Med Sci Monit In Press; DOI: 10.12659/MSM.942923  

10 Jan 2024 : Clinical Research  

Efficacy of Bakri Intrauterine Balloon in Managing Postpartum Hemorrhage: A Comparative Analysis of Vaginal...

Med Sci Monit In Press; DOI: 10.12659/MSM.943072  

15 Jan 2024 : Clinical Research  

Comparison of Modified Shock Index and Shock Index for Predicting Massive Transfusion in Women with Primary...

Med Sci Monit In Press; DOI: 10.12659/MSM.943286  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750