02 May 2003
Determination of intracellular NAD+ levels to study biological damage induced by pesticides
M. Faraone-Mennella, M. Malanga, A. Petrella, A. Ferone, C. Bufi, B. FarinaMed Sci Monit 2003; 9(1): 23-0 :: ID: 15073
Abstract
Exposure to environmental pollutants has been identified as one of the major causes of tumors in humans. Epidemiological data showed an increase of cancer incidence among farmers using pesticides. Different studies have shown that many of the compounds used in agriculture are able to interact with DNA, either directly or after metabolic activation, as well as other research evidenced that DNA structural alteration plays a primary role in neoplastic transformation, with implications of the poly(ADPribosyl)ation reaction.Increased intracellular levels of poly(ADPribose) and concomitant reduction of NAD+ content are typically found in cells undergoing genotoxic stress. Overactivation of PARP causes an extensive decrease of cellular NAD+ and ATP depletion, and induces cell necrosis.In our study, cellular NAD+ levels have been tested to define a new approach to evaluate the biological damage in a healthy population of farmers (in particular floriculturists) exposed routinely to the use of pesticides.Lymphocytes were prepared from peripheral blood of 100 subjects, either exposed or not exposed (controls) to pesticides and were used to monitor NAD+ levels according to classic procedures [1,2]. In parallel, aliquots of blood samples were subjected to conventional laboratory analyses to assess the health status at the time of recruitment. All the subjects answered also a questionnaire to collect individual and familiar anamnestic data.Here we present preliminary results obtained from this study, comparing the biochemical ones with epidemiological data. References: 1.Bernofsky C, Swan M: Anal Biochem, 1973; 53: 452 2.Jacobson EL, Jacobson MK: Meth Enzymol, 1997; 280: 221
Keywords: NAD+, Pesticides, farmer, Necrosis
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
06 Mar 2024 : Clinical Research
Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...Med Sci Monit In Press; DOI: 10.12659/MSM.941102
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952