02 May 2003
Med Sci Monit 2003; 9(1): 72- :: ID: 15215
Niacin deficiency in rats decreases bone marrow NAD+, impairing PARP function. Using the micronucleus (MN) assay, this study examined altered dietary niacin status on genomic stability in rats. Weanling male Long-Evans rats were fed niacin deficient (ND), or pair-fed niacin replete (PF) diet for 3 weeks. Bone marrow MN frequency in polychromatic erythrocytes (MN-PCEs) was increased significantly (4.4-fold) by deficiency alone. As the decrease in marrow NAD+ levels was dramatic (80%), the model was extended to include adolescent rats, using 2 diets which cause moderate and mild deficiencies. ND rats with a moderate decrease in marrow NAD+ (55%), showed a significant increase in MN-PCE (1.9-fold). However, ND rats with a mild decrease in NAD+ (25%) did not have a significantly enhanced MN-PCE frequency (1.2-fold). These experiments have identified the range of marrow NAD+ levels that lead to this type of genomic instability. To further characterize the interaction between niacin status and MN formation, ethylnitrosourea (ENU) treatment (30 mg/kg) was used to induce DNA damage. ND, in the weanling model, caused a dramatic increase (3.6-fold) in ENU-induced MN-PCEs (likely clastogenic in nature). This study emphasizes the importance of niacin in the maintenance of genomic stability in rats. We are working on identification of aneugenic versus clastogenic MN to help define the mechanisms by which ND increases genomic instability. Other data from our laboratory using comet analysis suggests that clastogenicity will not account for the large increase in genomic instability due to ND alone.
Keywords: niacin deficiency, NAD+, ethylnitrosourea (ENU), micronuclei (MN)
01 January 2023 : EditorialEditorial: Current Status of Two Adjuvanted Malaria Vaccines and the World Health Organization (WHO) Strategy to Eradicate Malaria by 2030
Med Sci Monit 2023; 29:e939357
26 January 2023 : EditorialEditorial: The XBB.1.5 (‘Kraken’) Subvariant of Omicron SARS-CoV-2 and its Rapid Global Spread
Med Sci Monit In Press; DOI: 10.12659/MSM.939580
19 January 2023 : Clinical ResearchEvaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...
Med Sci Monit In Press; DOI: 10.12659/MSM.938892
19 January 2023 : Clinical ResearchDetermining the Impact of the COVID-19 Pandemic on Dental Care in the Serbian Adult Population: An Online Q...
Med Sci Monit 2023; 29:e939238
30 Jan 2023 : Clinical ResearchSplenic Artery Steal Syndrome in Patients with Liver Cirrhosis: A Retrospective Clinical Study
Med Sci Monit In Press; DOI: 10.12659/MSM.938998
27 Jan 2023 : Database AnalysisAssociation Between Neutrophil-Lymphocyte Ratio and All-Cause Mortality in Critically Ill Patients with Chr...
Med Sci Monit In Press; DOI: 10.12659/MSM.938554
27 Jan 2023 : Clinical ResearchProposal for a Simple Equation for Limb Muscle Weight Calculation
Med Sci Monit In Press; DOI: 10.12659/MSM.938606
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292