Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 May 2003

Niacin deficiency enhances spontaneous and nitrosourea-induced micronuclei in rat bone marrow

L. Toxopeus, J. Kirkland

Med Sci Monit 2003; 9(1): 72-0 :: ID: 15215

Abstract

Niacin deficiency in rats decreases bone marrow NAD+, impairing PARP function. Using the micronucleus (MN) assay, this study examined altered dietary niacin status on genomic stability in rats. Weanling male Long-Evans rats were fed niacin deficient (ND), or pair-fed niacin replete (PF) diet for 3 weeks. Bone marrow MN frequency in polychromatic erythrocytes (MN-PCEs) was increased significantly (4.4-fold) by deficiency alone. As the decrease in marrow NAD+ levels was dramatic (80%), the model was extended to include adolescent rats, using 2 diets which cause moderate and mild deficiencies. ND rats with a moderate decrease in marrow NAD+ (55%), showed a significant increase in MN-PCE (1.9-fold). However, ND rats with a mild decrease in NAD+ (25%) did not have a significantly enhanced MN-PCE frequency (1.2-fold). These experiments have identified the range of marrow NAD+ levels that lead to this type of genomic instability. To further characterize the interaction between niacin status and MN formation, ethylnitrosourea (ENU) treatment (30 mg/kg) was used to induce DNA damage. ND, in the weanling model, caused a dramatic increase (3.6-fold) in ENU-induced MN-PCEs (likely clastogenic in nature). This study emphasizes the importance of niacin in the maintenance of genomic stability in rats. We are working on identification of aneugenic versus clastogenic MN to help define the mechanisms by which ND increases genomic instability. Other data from our laboratory using comet analysis suggests that clastogenicity will not account for the large increase in genomic instability due to ND alone.

Keywords: niacin deficiency, NAD+, ethylnitrosourea (ENU), micronuclei (MN)

Add Comment 0 Comments

Editorial

01 February 2024 : Editorial  

Editorial: Global Health Concerns as Vaccine-Preventable Infections Including SARS-CoV-2 (JN.1), Influenza, Respiratory Syncytial Virus (RSV), and Measles Continue to Rise

Dinah V. Parums

DOI: 10.12659/MSM.943911

Med Sci Monit 2024; 30:e943911

0:00

In Press

01 Feb 2024 : Review article  

Review of the Evaluation of Pulmonary Hypoplasia as an Important Determinant of Clinical Outcomes in Infant...

Med Sci Monit In Press; DOI: 10.12659/MSM.943259  

0:00

19 Feb 2024 : Clinical Research  

Diagnostic Accuracy of Ileocolic Artery and Vein Diameter for Acute Appendicitis

Med Sci Monit In Press; DOI: 10.12659/MSM.943846  

23 Feb 2024 : Clinical Research  

Early Mobilization in Pediatric Critical Care: Exploring the Gap Between Theory and Practice in Saudi Arabia

Med Sci Monit In Press; DOI: 10.12659/MSM.942467  

01 Feb 2024 : Review article  

Enhancing Patient Adherence to Newly-Prescribed Medicine for Chronic Diseases: A Comprehensive Review and C...

Med Sci Monit In Press; DOI: 10.12659/MSM.942923  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750