09 August 2004
Metallothionein overexpression does not protect against inflammatory bowel disease in a murine colitis modelHelieh S. Oz, Theresa Chen, Willem J.S. de Villiers, Craig J. McClain
Med Sci Monit 2005; 11(3): BR69-73 :: ID: 15372
Background: We evaluated whether modulation of metallothionein (MT, and subsequent oxidative stress) would infl uence the development and progression of colitis.
Material/Methods: MT-transgenic (MT-TG), MT-knockout (MT-KO), and wild-type (WT) mice were treated with Dextran Sodium Sulfate (DSS) to induce colitis compared to controls (no DSS).
Results: The DSS treated MT-TG and MT-KO mice responded in a manner similar to that of DSS treated WT mice, with all groups developing severe colitis. The colonic MT content in DSS treated animals increased drastically when compared to controls. The colonic and blood levels of the antioxidant, glutathione (GSH), were signifi cantly reduced in DSS treated MT-TG and MT-KO mice. However,
there was no signifi cant difference in the cysteine levels in these mice. The subcellular level of MT was highest in intestinal crypts loci of MT-TG mice and was not observed in MT-KO mice as well as in
the inflammatory lesions.
Conclusions: We conclude that MT does not appear to infl uence the development or progression of intestinal pathology in the DSS animal model of IBD.
Keywords: Colitis - chemically induced, Colitis - metabolism, Colitis - pathology, Inflammatory Bowel Diseases - metabolism, Colitis - pathology, Colon - pathology, Dextran Sulfate, Gene Expression, Inflammatory Bowel Diseases - pathology, Metallothionein - metabolism, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic
01 March 2023 : EditorialEditorial: Global Surveillance of Highly Pathogenic Avian Influenza Viruses in Poultry, Wild Birds, and Mammals to Prevent a Human Influenza Pandemic
Med Sci Monit 2023; 29:e939968
03 March 2023 : Database AnalysisReinfections from SARS-CoV-2: A Retrospective Study from the Gyncentrum Genetic Laboratory in Sosnowiec, Po...
Med Sci Monit In Press; DOI: 10.12659/MSM.939452
09 February 2023 : Clinical ResearchEvaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...
Med Sci Monit 2023; 29:e938892
19 January 2023 : Clinical ResearchDetermining the Impact of the COVID-19 Pandemic on Dental Care in the Serbian Adult Population: An Online Q...
Med Sci Monit 2023; 29:e939238
27 December 2022 : Clinical ResearchEffect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...
Med Sci Monit 2022; 28:e938926
19 Mar 2023 : Clinical ResearchManagement of Unstable C1 Fractures Involving the Lateral Mass: Posterior C1-C2 Screw-Rod Fixation and Fusion
Med Sci Monit In Press; DOI: 10.12659/MSM.938600
17 Mar 2023 : Clinical ResearchRelationship Between Autonomic Nervous System Activity and Axial Length in Children
Med Sci Monit In Press; DOI: 10.12659/MSM.939451
15 Mar 2023 : Clinical ResearchRelationship Between Psychological Symptoms and Self-Esteem Levels Before and After Stomach Botox Treatment
Med Sci Monit In Press; DOI: 10.12659/MSM.939618
15 Mar 2023 : Clinical ResearchShort-Term Patient Outcomes Using the Interlaminar Endoscopic Surgical System Ilessys® Delta System versus ...
Med Sci Monit In Press; DOI: 10.12659/MSM.938477
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952