Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 June 2005 : Case report  

Ex vivo expanded murine bone marrow cells with a multiple cytokine cocktail retain long-term hematopoietic reconstitution potentials.

Quan-en Yang, Steven E. Fong, Kungui Li, Kungui Li, Matthew A. Gonda, Gregory J. Tobin

Med Sci Monit 2005; 11(6): BR154-161 :: ID: 16477

Abstract

BACKGROUND: Although ex vivo expansion of bone marrow (BM) cells has been proposed as an effective method for the early recovery from pancytopenia in patients with bone marrow stem cell transplantation (BMT), the expansion potential and the long-term reconstitution capability of such BM cells is still controversial. We describe here a multiple cytokine medium (MCM) containing major hematopoietic stimulation factors and conditioned medium from PHA-stimulated murine spleen cells that permits the expansion of BM cells with long-term hematopoietic reconstitution capacity. MATERIAL/METHODS: Male murine BM cells were expanded in MCM for 4 to 14 days and injected into lethally irradiated syngeneic female mice. The mice were maintained for 18 months after transplantation for evaluation of hematopoietic reconstitution. RESULTS: The expanded cells contained pluripotent hematopoietic stem cells and lineage committed progenitors as well as terminally differentiated cells. They permitted full recovery of lethally irradiated mice in both early and late stages in same numbers equivalent to that of unexpanded cells. More than 80% of the progenitor cells were donor originated after 18 months. Expanded cells were able to be transduced with a retroviral vector expressing Beta-galactosidase, and continued to express the marker following BMT. CONCLUSIONS: With the use of MCM, the quantity of donor cells from BM and other sources might be greatly reduced. Ex vivo expanded BM cells might also facilitate gene manipulation in vitro by retroviral vectors.

Keywords: Bone Marrow Transplantation - physiology, Bone Marrow Cells - physiology, Bone Marrow Transplantation - physiology, Cell Division - drug effects, Colony-Forming Units Assay, Cytokines - pharmacology, DNA Primers, Hematopoiesis - physiology, Mice, Inbred BALB C, Phytohemagglutinins - pharmacology, Polymerase Chain Reaction, Spleen - drug effects, Y Chromosome

Add Comment 0 Comments

632 1

Editorial

01 June 2023 : Editorial  

Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.941209

Med Sci Monit 2023; 29:e941209

0:00

In Press

01 Jun 2023 : Clinical Research  

Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...

Med Sci Monit In Press; DOI: 10.12659/MSM.940472  

01 Jun 2023 : Clinical Research  

Internal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s Disease

Med Sci Monit In Press; DOI: 10.12659/MSM.940873  

01 Jun 2023 : Clinical Research  

Effectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...

Med Sci Monit In Press; DOI: 10.12659/MSM.940187  

01 Jun 2023 : Clinical Research  

Influence of Insulin Resistance on Diabetes Nephropathy

Med Sci Monit In Press; DOI: 10.12659/MSM.939482  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

0:00

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750