Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 July 2005 : Extended abstract from EFAS 2011  

Apoptotic efficacy and inhibitory effect of dexamethasone on matrix metalloproteinase.

Farshid Saadat, Mohammad Reza Khorramizadeh, Abbas Mirshafiey

Med Sci Monit 2005; 11(7): BR253-257 :: ID: 16978

Abstract

Background: Background: There is now accumulating evidence that matrixmetalloproteinases and apoptosis may play an important role in inflammation processes. This study wasundertaken to determine the effect of dexamethasone on apoptosis and matrix metalloproteinase 2 (MMP-2)activity compared with two nonsteroidal anti-inflammatory drugs (NSAIDs), piroxicam and diclofenac. Material/Methods:A fibrosarcoma (WEHI-164) cell line was used for evaluating tolerability, MMP-2 activity, and apoptosis.Dexamethasone, piroxicam, and diclofenac were used at concentrations of 10-200 microg/ml in triplicate,two-fold dilutions. MMP-2 activity was assessed using zymography. For assessment of apoptosis, terminaldeoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was used. Results: Cytotoxicityanalysis of dexamethasone showed a greater tolerability than diclofenac at concentrations of 10-100 microg/ml,whereas the cytotoxic effect of dexamethasone and piroxicam were parallel at doses of 5-80 microg/ml.The dose-dependent inhibitory effect of dexamethasone on MMP-2 activity was significantly less than thatof the tested NSAIDs at concentrations of 10-80 microg/ml, while it increased at doses of >100 microg/mlcompared with piroxicam. Moreover, the rate of apoptosis for dexamethasone-treated cells was 20.92%,the values for diclofenac and piroxicam being 78% and 28.15%, respectively. Conclusions: Our findingssuggest that dexamethasone is able to induce apoptosis and suppress MMP-2 activity. Collectively, dexamethasonemight be assumed as an agent which could be recommended for chemopreventive purposes.

Keywords: Dexamethasone - pharmacology, Diclofenac - pharmacology, Matrix Metalloproteinase 2 - antagonists & inhibitors, Piroxicam - pharmacology, Anti-Inflammatory Agents, Non-Steroidal - pharmacology, Antineoplastic Agents - pharmacology, Cell Survival - drug effects, Dexamethasone - pharmacology, Diclofenac - pharmacology, Fibrosarcoma, In Situ Nick-End Labeling, Matrix Metalloproteinase 2 - metabolism, Matrix Metalloproteinase Inhibitors, Piroxicam - pharmacology

0 Comments

Editorial

01 February 2023 : Editorial  

Editorial: The XBB.1.5 (‘Kraken’) Subvariant of Omicron SARS-CoV-2 and its Rapid Global Spread

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939580

Med Sci Monit 2023; 29:e939580

SARS-CoV-2/COVID-19

19 January 2023 : Clinical Research  

Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...

Med Sci Monit In Press; DOI: 10.12659/MSM.938892  

27 December 2022 : Clinical Research  

Effect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...

Med Sci Monit 2022; 28:e938926

10 December 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit 2022; 28:e938647

In Press

03 Feb 2023 : Clinical Research  

Treatment of Gingival Recession Defects with Pouch and Tunnel Technique Using Connective Tissue Graft and L...

Med Sci Monit In Press; DOI: 10.12659/MSM.938865  

02 Feb 2023 : Clinical Research  

Online Questionnaire-Based Study to Evaluate the Attitudes and Use of Rubber Dental Dams by Saudi Dental Pr...

Med Sci Monit In Press; DOI: 10.12659/MSM.938672  

02 Feb 2023 : Database Analysis  

Prognostic-Related Biomarkers in Pancreatic Ductal Adenocarcinoma Correlating with Immune Infiltrates Based...

Med Sci Monit In Press; DOI: 10.12659/MSM.938785  

01 Feb 2023 : Clinical Research  

Comparison of 3 Enzyme-Linked Immunoassay Methods to Evaluate Serum Concentrations of Infliximab and Antibo...

Med Sci Monit In Press; DOI: 10.12659/MSM.939084  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750