Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

15 May 2002

Induction of cytokine production in human T cells and monocytes by highly purifiedlipoteichoic acid: involvement of Toll-like receptors and CD14.

Douglas Golenbock, Daniel Foster, Terje Espevik, Christoph Thiemermann, Thomas Hartung, Andra Schromm, Trude Flo, Siegfried Morath, Espen Ellingsen, Rigmor Solberg, Ansgar Aasen, Jacob Wang

Med Sci Monit 2002; 8(5): BR149-156 :: ID: 420854

Abstract

BACKGROUND: The pro-inflammatory potential of lipoteichoic acid (LTA) fromStaphylococcus aureus is controversial. The present study was undertaken to examine the ability of highlypurified and characterized S. aureus LTA to stimulate the production of pro-inflammatory cytokines inhuman leukocytes at both mRNA and protein level, and to study the involvement of Toll-like receptors(TLRs) and CD14 in this response. MATERIAL/METHODS: Purified LTA was administered to whole human bloodex-vivo (or primary adherent monocytes) and the cytokine response assessed in plasma by EIA. CytokinemRNA was measured by RT-PCR on leukocyte subsets isolated following stimulation. To study the involvementof specific receptors for LTA signaling, CHO cells transfected with CD14 and/or TLR2, TLR4 were used,as well as antibodies directed against these receptors. RESULTS: Addition of highly purified LTA to humanwhole blood or primary adherent human monocytes elicited a time and concentration dependent release oftumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), IL-6 and IL-8. Messenger RNAencoding TNF-alpha, IL-1 beta and IL-6 seemed to be accumulated in monocytes and T cells, but not ingranulocytes and B cells. Expression of TLR2, but not TLR4, in chinese hamster ovary cells conferredresponsiveness to LTA. However, antibodies directed towards TLR2 (clone TL2.1) or TLR4 (clone HTA125)failed to inhibit TNF-a release induced by LTA in both the whole blood model and in adherent monocytes.In contrast, blockade of the CD14 receptor with MAb18D11 strongly attenuated the LTA induced releaseof TNF-alpha in both models. CONCLUSIONS: We propose that (i) LTA from S. aureus triggers the releaseof cytokines during staphylococcal infections, and (ii) both monocytes and T cells contribute to cytokineproduction induced by LTA. TLR2 may mediate cellular activation by LTA, but the functional significanceof this receptor during staphylococcal infections remains elusive.

Keywords: Actins, Antibodies, Monoclonal, Antigens, CD14, CHO Cells, Cell Adhesion, Cells, Cultured, Cytokines, Dose-Response Relationship, Drug, Drosophila Proteins, Hamsters, Kinetics, Membrane Glycoproteins, Monocytes, RNA, Messenger, Receptors, Cell Surface, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Teichoic Acids, Time Factors

Add Comment 0 Comments

588 22

Editorial

01 May 2023 : Editorial  

Editorial: Twenty Years On from Sequencing the Human Genome, Personalized/Precision Oncology Prepares to Meet the Challenges of Checkpoint Inhibitor Therapy

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.940911

Med Sci Monit 2023; 29:e940911

0:00

In Press

31 May 2023 : Review article  

Prevalence, Diagnosis, and Management of Eclampsia and the Need for Improved Maternal Care: A Review

Med Sci Monit In Press; DOI: 10.12659/MSM.939919  

30 May 2023 : Clinical Research  

A Case-Control Study of Cognitive Function in Patients with End-Stage Renal Disease Before and After Hemodi...

Med Sci Monit In Press; DOI: 10.12659/MSM.940409  

30 May 2023 : Review article  

A Review of the Role of the Zebrafish (Danio reiro) in Preclinical and Clinical Models of Biomarker Identif...

Med Sci Monit In Press; DOI: 10.12659/MSM.940550  

30 May 2023 : Database Analysis  

The COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland

Med Sci Monit In Press; DOI: 10.12659/MSM.940904  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

0:00

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750