11 March 2002
Characterization of Clostridium perfringens strains isolated from Polish patients with suspected antibiotic-associated diarrhea.
Piotr Obuch-Woszczatyński, Mirosław Łuczak, Willem Van Leeuwen, Alex van Belkum, Nicole van den Braak, Hanna Pituch, Henri Verbrugh, Felicja Meisel-Mikołajczyk, Gayane MartirosianMed Sci Monit 2002; 8(3): BR85-88 :: ID: 420915
Abstract
BACKGROUND: The aim of our research was to investigate the role of enterotoxin-producing anaerobic bacteria other than Clostridium difficile in the etiology of antibiotic-associateddiarrhea. This article presents data related to C. perfringens. MATERIAL/METHODS: Stool samples takenfrom 158 patients with suspected antibiotic-associated diarrhea were specifically cultured for Clostridiumdifficile, Bacteroides fragilis and Clostridium perfringens. In order to associate the presence of virulencefactors in the bacterial isolates thus collected with disease features, all strains were geneticallyand phenotypically analyzed for toxin production. All isolated C. perfringens strains were cultured inEllner sporulation-promoting medium. RESULTS: In 21 of the 158 patients (13%) C. perfringens could becultivated from the fecal specimen. None of the strains produced enterotoxin, and consequently the cpegene was not detected by PCR in any of these strains. C. perfringens and C. difficile were cultivatedfrom the same stool samples in 4 cases. Interestingly, in one case toxin A-negative/toxin B positiveC. difficile and non-enterotoxigenic C. perfringens were co-cultured. After application of a heat shock(100 degrees C at 30 min.) only two C. perfringens strains producing thermoresistant spores were detected.Pulsed field gel electrophoresis (PFGE) demonstrated genetic heterogenicity among the C. perfringensstrains, suggesting that these bacteria were already presented upon hospital admission. CONCLUSIONS:It seems unlikely that nosocomial transfer has taken place. The relatively low incidence suggests thatC. perfringens is not a major primary cause of antibiotic-associated diarrhea.
Keywords: Anti-Bacterial Agents, Bacteroides fragilis, Clostridium perfringens, diarrhea, Enterotoxins, Feces, Phylogeny, Poland, Research Support, Non-U.S. Gov
Editorial
01 February 2023 : Editorial
Editorial: The XBB.1.5 (‘Kraken’) Subvariant of Omicron SARS-CoV-2 and its Rapid Global SpreadDOI: 10.12659/MSM.939580
Med Sci Monit 2023; 29:e939580
SARS-CoV-2/COVID-19
19 January 2023 : Clinical Research
Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...Med Sci Monit In Press; DOI: 10.12659/MSM.938892
19 January 2023 : Clinical Research
Determining the Impact of the COVID-19 Pandemic on Dental Care in the Serbian Adult Population: An Online Q...Med Sci Monit 2023; 29:e939238
27 December 2022 : Clinical Research
Effect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...Med Sci Monit 2022; 28:e938926
10 December 2022 : Clinical Research
Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...Med Sci Monit 2022; 28:e938647
In Press
01 Feb 2023 : Clinical Research
Comparison of 3 Enzyme-Linked Immunoassay Methods to Evaluate Serum Concentrations of Infliximab and Antibo...Med Sci Monit In Press; DOI: 10.12659/MSM.939084
31 Jan 2023 : Clinical Research
Balloon Dilation Eustachian Tuboplasty Combined with Tympanotomy Tube Insertion for Treatment of Refractory...Med Sci Monit In Press; DOI: 10.12659/MSM.938879
31 Jan 2023 : Clinical Research
Comparison of Vision Correction and Corneal Thickness at 180-Day Follow-Up After Femtosecond Laser-Assisted...Med Sci Monit In Press; DOI: 10.12659/MSM.939099
30 Jan 2023 : Clinical Research
Splenic Artery Steal Syndrome in Patients with Liver Cirrhosis: A Retrospective Clinical StudyMed Sci Monit In Press; DOI: 10.12659/MSM.938998
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952