22 February 2002
A prolonged time interval between blood sample collection and centrifugationcauses an increase in serum carbohydrate-deficient transferrin.
Torsten Arndt, Jürgen KropfMed Sci Monit 2002; 8(2): BR61-64 :: ID: 420946
Abstract
BACKGROUND: Carbohydrate-deficient transferrin (CDT) is used for the laboratorydiagnosis of chronic alcohol abuse. Non-optimal preanalysis can cause an increase in CDT and false positiveresults. The aim of our study was to determine whether CDT results change over time between collectionof the blood sample and centrifugation, and whether shipment of whole blood samples is a potential sourceof false positive CDT reports. MATERIAL/METHODS: 152 blood samples were drawn from 38 persons (4 tubesper person, one venipuncture) and randomly assigned to 4 groups with different time intervals betweenblood sample collection and centrifugation (1h, 24h, 48h, 144h). CDT analysis was done using the ChronAlcoI.D.assay. The statistical analysis was based on box-plots, ANOVA and Kruskal-Wallis ANOVA. RESULTS: Themeans and medians of CDT increased with the time of whole blood storage. ANOVA analysis of between-groupdifferences was significant for mean CDT concentrations between 1 and 144 hours of whole blood storage.There was no correlation between CDT and free hemoglobin as a measure of hemolysis. An interference ofhemolysis with CDT measurement can be excluded as the main cause of increased CDT results with wholeblood storage time. Whether an in vitro degradation of the transferrin N-glycan chains causes the CDTincrease should be evaluated by isoelectric focusing of the transferrin isoforms in a further study.CONCLUSIONS: Storage or shipment of whole blood samples can shift initially normal CDT values to borderlineand borderline to pathological CDT results.
Keywords: Blood, Centrifugation, False Positive Reactions, Phlebotomy, Specimen Handling, Time Factors, Transferrin
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
08 Mar 2024 : Clinical Research
Evaluation of Foot Structure in Preschool Children Based on Body MassMed Sci Monit In Press; DOI: 10.12659/MSM.943765
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952