01 September 2005
Isolation and characterization of unserotypable lysogens of Vibrio choleraephage PS166.Siddhartha Basu, Ranajit K. Ghosh
Med Sci Monit 2005; 11(9): BR335-342 :: ID: 428445
Background: Temperate phages of Vibrio cholerae are the major force inthe emergence of newer pathogenic clones. PS166, a temperate vibriophage, has the ability to convertpathogenic biotypes of the V. Cholerae O1 serogroup. During the conversion process, PS166 also tendsto produce a group of lysogens neither reported nor characterized so far. Characterization of those lysogensmay provide insight into the emergence of newer pathogenic clones of V. Cholerae. Material/Methods: TheV. cholerae eltor strain MAK757 was used to generate PS166 lysogens which were serotyped by using antiseraspecific to V. Cholerae O1 and O139 strains. Multiplex PCR was used to compare the status of variouspathogenic markers of those lysogens. The outer membrane proteins (OMP) of the lysogens were determinedby SDS-PAGE. Results: Temperate phage PS166 infection of V. cholerae eltor MAK757 gave rise to a newkind of lysogens termed unserotypable due to their lack of reactivity to both O1 and O139 antisera. Moreover,these lysogens displayed a new phage sensitivity profile different from both classical and parental eltorbiotypes. The genetic profiles of the CTX element and VPI region of these lysogens were found to be unaltered,together with the biotype-specific markers mshA and hlyA. Though the genetic loci of major outer membraneproteins did not change after lysogenization, the outer membrane protein profile of these lysogens showedthe absence of a 40-kDa band corresponding to the toxR-controlled porin, ompT. Conclusions: We provideevidence for the vibriophage PS166-mediated lysogenic conversion of pathogenic cholera strains.
Keywords: DNA, Bacterial - genetics, Base Sequence, Bacteriophages - pathogenicity, Genes, Bacterial, Genetic Markers, Lysogeny - genetics, Serotyping, Vibrio cholerae - virology, Vibrio cholerae O1 - virology, Vibrio cholerae non-O1 - virology
01 May 2023 : EditorialEditorial: Twenty Years On from Sequencing the Human Genome, Personalized/Precision Oncology Prepares to Meet the Challenges of Checkpoint Inhibitor Therapy
Med Sci Monit 2023; 29:e940911
28 May 2023 : Database AnalysisScientometric and Visualized Analysis of Intravoxel Incoherent Motion Magnetic Resonance Imaging: 1988-2021
Med Sci Monit In Press; DOI: 10.12659/MSM.938715
25 May 2023 : Database AnalysisThe COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland
Med Sci Monit In Press; DOI: 10.12659/MSM.940904
25 May 2023 : Review articleA Review of Preclinical and Clinical Studies in Support of the Role of Non-Steroidal Anti-Inflammatory Drug...
Med Sci Monit In Press; DOI: 10.12659/MSM.940635
25 May 2023 : Clinical ResearchPeriapical Lesions and Missed Canals in Endodontically Treated Teeth: A Cone-Beam Computed Tomographic Stud...
Med Sci Monit In Press; DOI: 10.12659/MSM.940533
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952