01 October 2005
Lack of effect of hyperoxia on photodynamic therapy and lipid peroxidation in three different cancer cell linesAstrid Hjelde, Odrun A. Gederaas, Hans E. Krokan, Alf O. Brubakk
Med Sci Monit 2005; 11(10): BR351-356 :: ID: 430288
Background:The use of photodynamic therapy (PDT) to treat malignant tumors involves a photosensitizing drug, light, and oxygen. Since PDT requires oxygen, the purpose of this study was to investigate whether increased oxygen tension increases cell death compared with normoxic conditions (21 kPa oxygen).Material/Methods:The effect of hyperoxia on PDT and lipid peroxidation was investigated in two human colon carcinoma cell lines, SW480 and WiDr, and one rat bladder cell carcinoma, AY-27. The cells were incubated with 2 mM 5-aminolaevulinic acid (5-ALA) for 3.5 h at 37°C. This treatment induces the accumulation of protoporphyrin IX (PpIX), which is a potent photosensitizer. PpIX absorbs light energy and produces singlet oxygen in the presence of molecular oxygen, which then destroys the cancer cells. The cells were illuminated (0.5–30 min) at room temperature by blue light (435 nm) prior to hyperoxia exposure. This treatment was performed in a small, temperature-controlled (37°C) hyperbaric chamber using oxygen at a pressure of 100, 200, 300, or 400 kPa.Results:PDT performed under normoxia induced lipid peroxidation and caused a considerable decrease in cell survival. However, this decrease was not influenced by the presence of hyperoxia (P>0.05). Furthermore, hyperoxic exposure (400 kPa O[sub]2[/sub]) alone caused no increase in lipid peroxidation compared with untreated control cells.
Keywords: Aminolevulinic Acid - administration & dosage, Colonic Neoplasms - pathology, Hyperoxia - metabolism, Lipid Peroxidation, Photochemotherapy, Photosensitizing Agents - administration & dosage, Protoporphyrins - administration & dosage, Urinary Bladder Neoplasms - pathology
01 July 2022 : EditorialEditorial: World Health Organization (WHO) Variants of Concern Lineages Under Monitoring (VOC-LUM) in Response to the Global Spread of Lineages and Sublineages of Omicron, or B.1.1.529, SARS-CoV-2
Med Sci Monit 2022; 28:e937676
27 June 2022 : Clinical ResearchShoulder Injury Related to Vaccine Administration (SIRVA) in 16 Patients Following COVID-19 Vaccination Who...
Med Sci Monit In Press; DOI: 10.12659/MSM.937430
04 June 2022 : Clinical ResearchScreening for SARS-CoV-2 Infection in Students at the Medical University of Warsaw, Poland Between November...
Med Sci Monit 2022; 28:e936962
30 May 2022 : Clinical ResearchEffects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...
Med Sci Monit 2022; 28:e936069
01 Jul 2022 : Clinical ResearchDoes Early-Period Patient Dissatisfaction Turn Into Satisfaction Over Time After Total Knee Replacement?
Med Sci Monit In Press; DOI: 10.12659/MSM.936792
30 Jun 2022 : Laboratory ResearchBcl-2 19-kDa Interacting Protein 3 (BNIP3)-Mediated Mitophagy Attenuates Intermittent Hypoxia-Induced Human...
Med Sci Monit In Press; DOI: 10.12659/MSM.936760
30 Jun 2022 : Clinical ResearchAntioxidant System and Ions Concentrations After Femoral Neck and Resurfacing Hip Arthroplasty
Med Sci Monit In Press; DOI: 10.12659/MSM.936335
Most Viewed Current Articles
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788