10 April 2006
Derivation of embryonic stem cells for cellular therapy: Challenges and newstrategies.
Philippe TaupinMed Sci Monit 2006; 12(4): RA75-78 :: ID: 448936
Abstract
Cellular therapy is the replacement of unhealthy or damaged cells or tissuesby new ones. Embryonic stem (ES) cells are undifferentiated cells that can generate all the cell typesof the body, and therefore hold the potential to cure a broad range of diseases and injuries, rangingfrom diabetis, liver and heart diseases, to neurological diseases, such as Alzheimer's and Parkinson'sdiseases. The derivation of human ES (hES) cells has been a major step toward bringing ES cell researchto therapy. However, there are several challenges to the advent of ES cell research to therapy. Amongthem, the derivation of hES cell lines devoid of animal contaminants, the maintenance of their normalkaryotypes, their potentials to form tumors upon grafting, and the derivation of isogenic hES cell lines.Stringent ethical and political guidelines are also limiting the use of human embryos for research, therebylimiting progress in ES cell research. Recently, several investigators have devised protocols to derivehES cells free of feeder layer and animal serum, reported that some established cell lines remain stableovertime, pre-differentiated ES cells in vitro to circumvent the risk of tumor formation, and derivedES cell lines without destroying embryos. In this manuscript, we will review and discuss these developmentsthat may unlock ES cell research and therapy.
Keywords: Cell Differentiation, Cell Line, Cloning, Organism - ethics, Nuclear Transfer Techniques, Stem Cells - cytology
Editorial
01 January 2023 : Editorial
Editorial: Current Status of Two Adjuvanted Malaria Vaccines and the World Health Organization (WHO) Strategy to Eradicate Malaria by 2030DOI: 10.12659/MSM.939357
Med Sci Monit 2023; 29:e939357
SARS-CoV-2/COVID-19
19 January 2023 : Clinical Research
Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...Med Sci Monit In Press; DOI: 10.12659/MSM.938892
19 January 2023 : Clinical Research
Determining the Impact of the COVID-19 Pandemic on Dental Care in the Serbian Adult Population: An Online Q...Med Sci Monit 2023; 29:e939238
27 December 2022 : Clinical Research
Effect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...Med Sci Monit 2022; 28:e938926
10 December 2022 : Clinical Research
Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...Med Sci Monit 2022; 28:e938647
In Press
20 Jan 2023 : Clinical Research
A Study of 42 Partially Edentulous Patients with Single-Crown Restorations and Implants to Compare Bone Los...Med Sci Monit In Press; DOI: 10.12659/MSM.939225
20 Jan 2023 : Clinical Research
Perfusate Neutrophil Gelatinase-Associated Lipocalin, Kidney Injury Molecular-1, Liver-Type Fatty Acid Bind...Med Sci Monit In Press; DOI: 10.12659/MSM.938758
19 Jan 2023 : Clinical Research
Bakri Balloon for Treatment of Postpartum Hemorrhage: A Real-World 2016-2020 Study in 279 Women from a Sing...Med Sci Monit In Press; DOI: 10.12659/MSM.938823
19 Jan 2023 : Clinical Research
Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...Med Sci Monit In Press; DOI: 10.12659/MSM.938892
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952