Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

23 April 2003

The structural abnormality of myeloma immunoglobulins tested by Congo red binding.

Paweł Spólnik, Barbara Piekarska, Barbara Stopa, Leszek Konieczny, Grzegorz Zemanek, Janina Rybarska, Marcin Król, Mateusz Nowak, Irena Roterman

Med Sci Monit 2003; 9(4): BR145-153 :: ID: 4702

Abstract

BACKGROUND: Frequently observed structural deviations of myeloma-derived immunoglobulins affect polypeptide chain packing and domain stability, enhancing their tendency to aggregate, with all the clinical consequences. Congo red complexation with myeloma immunoglobulins is proposed in this work as a general test to disclose the instability of these proteins. The large ribbon-like supramolecular ligands of Congo red form complexes with proteins by adhesion to ß-conformation polypeptide chains, if allowed to make contact with their backbone interfaces. This can occur in the case of myeloma-derived immunoglobulins with deficient polypeptide chain packing. MATERIAL/METHODS: Specially adapted two-dimensional agarose electrophoresis of serum proteins, which allows the transient contact of Congo red and serum proteins during migration, was used to reveal the presence of protein components amenable to ligand penetration and binding. The combination of electrophoresis and Congo red binding to proteins permits the removal of loosely attached dye and evaluation of the effective complexation properties of the immunoglobulin fraction directly in the serum. RESULTS: Comparative studies of dye complexation with two L chains having different reactivities with Congo red confirmed that dye binding depended on protein instability in the conditions used. Myeloma proteins revealed different binding capabilities in the test used here. CONCLUSIONS: The complexes formed by the supramolecular dye Congo red with myeloma immunoglobulins differ in stability. Those of high stability indicate the abnormal protein structure thought to produce clinical symptoms. This work proposes an easy technique to differentiate the stability of complexes.

Keywords: Coloring Agents - metabolism, Congo Red - metabolism, Immunoglobulin lambda-Chains - chemistry, Myeloma Proteins - chemistry

Add Comment 0 Comments

Editorial

01 April 2024 : Editorial  

Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug Resistance

Dinah V. Parums

DOI: 10.12659/MSM.944600

Med Sci Monit 2024; 30:e944600

0:00

In Press

06 Mar 2024 : Clinical Research  

Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...

Med Sci Monit In Press; DOI: 10.12659/MSM.941102  

06 Mar 2024 : Clinical Research  

Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943884  

08 Mar 2024 : Clinical Research  

Evaluation of Foot Structure in Preschool Children Based on Body Mass

Med Sci Monit In Press; DOI: 10.12659/MSM.943765  

15 Apr 2024 : Laboratory Research  

The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in Osteoarthritis

Med Sci Monit In Press; DOI: 10.12659/MSM.943738  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750