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30 January 2007

Esophageal muscle cell interaction with biopolymers

Mevlit Korkmaz, Tahsin Yakut, Adnan Narci, B Haluk Güvenç, Tuna Gülten, Murat Yağmurca, Barbaros Yiğit, Ayhan Bilir

Med Sci Monit 2007; 13(2): BR46-49 :: ID: 473724

Abstract

Background: The in vitro interactions of esophageal smooth muscle cells (SMCs) with synthetic absorbable polymers were tested and artifi cial muscle tissues harvested from subcutaneous implantation were examined.
Material/Methods: Esophageal tissue samples from adult and fetal (25-day gestational age) rabbits were cut into small pieces and cultured in Dulbecco’s Modifi ed Eagle Medium supplemented with 10% fetal bovine
serum. Growing cells were identifi ed as SMCs by immunostaining for anti-actin and anti-myosin antibodies. Equal volumes of agar gel and medium were mixed and used for 3-D culture. 5×10[sup]5[/sup] cells and 1 mg polyglycolic acid (PGA) and poly-lactide-co-glycolide acid (PLGA) fi bers were seeded in six-well tissue culture plates. On days 2 and 7 growing cells were counted by a hemocytometer and cell-polymer interactions were evaluated with light microscopy. Adult and fetal SMCs were seeded
onto the PGA and PLGA scaffolds, cultivated for two weeks, and implanted subcutaneously on the backs of the rabbits. Cell-polymer implants were retrieved after four weeks and muscle formation was evaluated histologically and immunohistochemically.
Results: Growing cells stained positive for actin and myosin proteins. Cell–polymer interactions were poor after 24 hours, whereas intensive attachment to the fi bers was detected 48 hours following cultivation. Both fi ber materials supported cell proliferation. PLGA scaffolds improved muscle formation
more effi ciently than PGA, and fetal and adult SMCs showed similar mass quality.
Conclusions: Scaffolds are important as cell-carrying vehicles, and material-cell interactions should be tested before application. A 3-D culture prepared with agar gel and medium is practical for testing material toxicity.

Keywords: Biopolymers - metabolism, Esophagus - metabolism, Lactic Acid - metabolism, Myocytes, Smooth Muscle - metabolism, Polyglycolic Acid - metabolism, Polymers - metabolism, Tissue Engineering

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