30 March 2007
Preliminary evaluation of the capacity of Surface-Active Phospholipids to provide semipermeability in a saline filtration environment
Yi Chen, Ross W. Crawford, Adekunle OloyedeMed Sci Monit 2007; 13(4): BR101-105 :: ID: 480303
Abstract
Background:Semipermeability to fluid transport is one of the principal attributes of a tissue like articular cartilage. Consequently, this characteristic can be exploited in attempts to understand the functional roles of the biological layer of Surface Active Phospholipids (SAPL) which form on its surfaces. A previous study, relevant to peritoneal SAPL was carried out in which hypertonic glucose solution was dialysed against physiological saline through SAPL membrane and concluded that SAPL possessed semipermeability. Our analysis extends this previous study by dialysing hypertonic and hypotonic saline solutions against physiological saline via SAPL membranes which is more relevant to the articular joint environment.
Material/Methods:Membranes were produced from either synthetic or bovine cartilage SAPL and used to carry out tests involving the dialysis of hypotonic and hypertonic sodium chloride solutions against physiological saline, using an Ussing chamber to hold both the membranes and dialysis fluids.
Results:The dialysis produced osmotic pressures which are commensurate with our experimental constraints, but strongly indicated that it is indeed possible to generate osmotic pressures using SAPL membranes, indicating the semipermeability of this lipid structure.
Conclusions:It is widely accepted that the collagen-proteoglycan membrane provides the semipermeability of articular cartilage despite the low levels of osmotic pressure recorded in our experiments, our results demonstrate that SAPL aggregation can constitute a semipermeable layer with a strong capability to contribute to the semipermeablity of the collagen-proteoglycan system especially on the surface of the tissue. Consequently its deficiency, as seen in osteoarthritis could lead/contribute to cartilage dysfunction.
Keywords: Cartilage - metabolism, 1,2-Dipalmitoylphosphatidylcholine - metabolism, Cattle, Dialysis, Filtration, Membranes, Artificial, Osmotic Pressure, Permeability, Phospholipids - metabolism, Sodium Chloride, Surface-Active Agents - metabolism
510 0
Editorial
01 June 2023 : Editorial
Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic PreparednessDOI: 10.12659/MSM.941209
Med Sci Monit 2023; 29:e941209
In Press
08 Jun 2023 : Clinical Research
Risk Factors for New Vertebral Compression Fracture After Percutaneous Vertebral Augmentation: A Retrospect...Med Sci Monit In Press; DOI: 10.12659/MSM.940134
08 Jun 2023 : Clinical Research
A Nomogram for Identifying HR+/Her2- Breast Cancer Patients with Positive Sentinel Lymph Nodes and Omitted ...Med Sci Monit In Press; DOI: 10.12659/MSM.940124
08 Jun 2023 : Clinical Research
Burden of COVID-19 on Mental Health of Resident Doctors in PolandMed Sci Monit In Press; DOI: 10.12659/MSM.940208
08 Jun 2023 : Clinical Research
Risk Prediction for Rapidly Progressive Interstitial Lung Disease in Anti-MDA5-Positive Dermatomyositis: Th...Med Sci Monit In Press; DOI: 10.12659/MSM.940251
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952