31 May 2007
Immunomodulatory and apoptotic effects of Stachys obtusicrena on proliferative lymphocytes
Zahra Amirghofran, Masoud Bahmani, Abbas Azadmehr, Katayoun JavidniaMed Sci Monit 2007; 13(6): BR145-150 :: ID: 484105
Abstract
Background: Several medicinal plants have shown anti-proliferative and apoptotic effects on human lymphocytes. In the present study the immunomodulatory properties of Stachys obtusicrena, a native plant to Iran, was examined and its possible effects on the induction of apoptosis were determined.
Material/Methods:The in vivo effect of the methanolic extract of S. obtusicrena on delayed type hypersensitivity (DTH) and antibody production was investigated in mice immunized with the antigen. The proliferation of human lymphocytes stimulated with mitogen in the presence of the extract was determined by [[sup]3[/sup]H]-thymidine incorporation assay. Mitogen-treated lymphocytes were exposed to the extract and analyzed for induction of apoptosis using flow cytometry and gel electrophoresis.
Results: Data obtained from the DTH and antibody responses indicated a dose-related decrease in both parameters in the extract-treated mice compared with the untreated control group. In the in vitro study performed on the lymphocytes, the extract caused a dose-dependent decline in [[sup]3[/sup]H]-thymidine uptake. The stimulation index of all cultures treated with different concentrations of the extract was less than 1. IL-2 levels in the culture supernatants of the activated lymphocytes showed a dose-related decrease as well. Results of flow cytometry indicated a dose-dependent accumulation of cells in the sub-G1 phase. The internucleosomal DNA fragmentation study showed typical DNA laddering in agarose gels.
Conclusions: The present study showed inhibitory effects of S. obtusicrena on both cellular and humoral immune responses and suggests that this effect may in part be due to the induction of apoptosis in proliferative lymphocytes.
Keywords: Stachys - immunology, Skin Tests, Plant Extracts - pharmacology, Phytohemagglutinins - pharmacology, Lymphocytes - drug effects, Interleukin-2 - metabolism, Hypersensitivity, Delayed - pathology, Dose-Response Relationship, Immunologic, DNA Fragmentation - drug effects, Cell Proliferation - drug effects, Antibody Formation - immunology, Antibody Formation - immunology, Stachys - immunology, Skin Tests, Plant Extracts - pharmacology, Phytohemagglutinins - pharmacology, Lymphocytes - drug effects, Interleukin-2 - metabolism, Hypersensitivity, Delayed - pathology, Dose-Response Relationship, Immunologic, DNA Fragmentation - drug effects, Cell Proliferation - drug effects
521 5
Editorial
01 June 2023 : Editorial
Editorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic PreparednessDOI: 10.12659/MSM.941209
Med Sci Monit 2023; 29:e941209
In Press
01 Jun 2023 : Clinical Research
Root Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...Med Sci Monit In Press; DOI: 10.12659/MSM.940472
01 Jun 2023 : Clinical Research
Internal Orifice Alloy Closure: A New Procedure for Treatment of Perianal Fistulizing Crohn’s DiseaseMed Sci Monit In Press; DOI: 10.12659/MSM.940873
01 Jun 2023 : Clinical Research
Effectiveness of Needle Aspiration versus Surgical Excision for Symptomatic Synovial Cysts of the Hip: A Si...Med Sci Monit In Press; DOI: 10.12659/MSM.940187
01 Jun 2023 : Clinical Research
Influence of Insulin Resistance on Diabetes NephropathyMed Sci Monit In Press; DOI: 10.12659/MSM.939482
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952