21 November 2002
Med Sci Monit 2002; 8(11): BR471-480 :: ID: 4843
BACKGROUND: The development of T-cell responses in natural hosts, such as cattle, would be a useful model of tuberculosis to investigate the changing pattern of T-cell responses and mycobacterial antigens recognized along with the progression of the disease. MATERIAL/METHODS: The present study was undertaken to characterize immune-effector cells and cytokines in Mycobacterium bovis AN5-sensitized cattle, from day 0 to 45 weeks post inoculation (PI). In vitro lymphoproliferation, cytotoxic T lymphocyte (CTL) response, and cytokine release pattern (viz. IL-2, IFN-gamma) from activated PBMC were assessed throughout the spectrum. The effort was made to identify various T-cell target antigens during different post sensitization stages, using T-cell immunoblotting. RESULTS: Cell-mediated immune (CMI) responses were very high between the 9th and 20th week PI. The early appearance of gd+ T-cells was followed by the predominance of CD4+ T-cells, and finally CD8+ T-cells until the 20th week PI. Lymphocyte-effector activity in terms of lymphoproliferation and CTL responses were quite evident between the 9th and 20th weeks PI and around the 12th week PI, respectively. Certain immunodominant polypeptides of culture filtrate preparation of molecular weights 72-74, 30-32, 25 & 22-24 kDa induced higher lymphoproliferation and CTL responses. The kinetics of in vitro cytokine release (IL-2 and IFN-gamma) from PBMC showed a definite trend in all M. bovis AN5-sensitized cattle, with a high concentration between the 9th and 20th weeks PI. CONCLUSIONS: The present investigation revealed some basic aspects of the immune-effector mechanism pertaining to bovine tuberculosis.
Keywords: Mycobacterium bovis - metabolism, Tuberculosis, Bovine - immunology, Tuberculosis, Bovine - microbiology
01 November 2023 : EditorialEditorial: Factors Driving New Variants of SARS-CoV-2, Immune Escape, and Resistance to Antiviral Treatments as the End of the COVID-19 Pandemic is Declared
Med Sci Monit 2023; 29:e942960
28 Nov 2023 : Clinical ResearchImpact of Fluorodeoxyglucose-Positron Emission Tomography/Computed Tomography on Therapeutic Decisions and ...
Med Sci Monit In Press; DOI: 10.12659/MSM.942122
28 Nov 2023 : Clinical ResearchLong-Term Outcomes of Decompression and Grafting in Acute Pathological Proximal Femur Fractures in Children...
Med Sci Monit In Press; DOI: 10.12659/MSM.943031
27 Nov 2023 : Clinical ResearchComparison of Outcomes from Emergency Admissions to a Major Trauma Center in Turkey of 1646 Elderly Patient...
Med Sci Monit In Press; DOI: 10.12659/MSM.942916
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
14 Dec 2022 : Clinical ResearchPrevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels
Med Sci Monit 2022; 28:e937990
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292