Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 May 1997

5' NC region of the HCV genome complexity detected by SSCP and RFLP analysis

Ilona Bednarek, Urszula Mazurek, Ewa Moric, Oksana Kowalczuk, Dorota Domal-Kwiatkowska, Adam Wilczok, Edyta Czechowska, Ewa Laskowska-Lepiarczyk, Zbigniew Gonciarz, Tadeusz Wilczok

Med Sci Monit 1997; 3(3): BR328-335 :: ID: 501072

Abstract

Comparison between different HCV isolates from different host pointed to the region-depended variability of HCV. There are several advantages in using the 5' NC region of the HCV genome for genotyping and reflecting quasispecies nature of the HCV. The aim of the presented study was to establish the degree of complexity of 5' NC region HCV genome and grouping of hepatitis C isolates by restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP) by the use of reverse - transcription - polymerase chain reaction products (RT-PCR). Representatives were checked by line probe assay. Isolated RNA preparations from whole blood or serum samples obtained from 55 patients with chronic HCV infection were used for cDNA synthesis. The nested polymerase chain reaction, RFLP experiments, SSCP analysis, the line probe assay and the phylogenetic analysis have shown that the applied methods RFLP and SSCP applied simultaneously to the analysis of the 5' NC virus region provided good evidence, that the region is highly complex. This phenomenon was examined by INNO-LIPA HCV II tests, used normally for genotyping, and it was shown, that the applied RFLP and SSCP methods alone are sufficient to describe the heterogeneity of this region.

Keywords: hepatitis C virus (HCV), Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP), genetic heterogeneit

Add Comment 0 Comments

Editorial

01 April 2025 : Editorial  

Editorial: Rapid Testing for the Avian Influenza A(H5N1) Virus is Urgently Required as Infections in Poultry and Dairy Cows are on the Rise, and so is Transmission to Humans

Dinah V. Parums

DOI: 10.12659/MSM.949109

Med Sci Monit 2025; 31:e949109

0:00

In Press

Clinical Research  

Serum Prolidase and Ischemia-Modified Albumin Levels in Neural Tube Defects: A Comparative Study of Myelome...

Med Sci Monit In Press; DOI: 10.12659/MSM.947873  

0:00

Clinical Research  

Impact of Depression, Fatigue, and Pain on Quality of Life in Slovak Multiple Sclerosis Patients

Med Sci Monit In Press; DOI: 10.12659/MSM.947630  

Clinical Research  

Longitudinal Evaluation of Metabolic Benefits of Inactivated COVID-19 Vaccination in Diabetic Patients in T...

Med Sci Monit In Press; DOI: 10.12659/MSM.947450  

0:00

Clinical Research  

Impact of Voltage Level on Hospitalization and Mortality in Electrical Injury Cases: A Retrospective Analys...

Med Sci Monit In Press; DOI: 10.12659/MSM.947675  

Most Viewed Current Articles

17 Jan 2024 : Review article   8,000,435

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

16 May 2023 : Clinical Research   702,963

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Mar 2024 : Editorial   30,040

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...

DOI :10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

28 Jan 2024 : Review article   23,978

A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and Future

DOI :10.12659/MSM.943912

Med Sci Monit 2024; 30:e943912

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750