01 May 1997
5' NC region of the HCV genome complexity detected by SSCP and RFLP analysis
Ilona Bednarek, Urszula Mazurek, Ewa Moric, Oksana Kowalczuk, Dorota Domal-Kwiatkowska, Adam Wilczok, Edyta Czechowska, Ewa Laskowska-Lepiarczyk, Zbigniew Gonciarz, Tadeusz WilczokMed Sci Monit 1997; 3(3): BR328-335 :: ID: 501072
Abstract
Comparison between different HCV isolates from different host pointed to the region-depended variability of HCV. There are several advantages in using the 5' NC region of the HCV genome for genotyping and reflecting quasispecies nature of the HCV. The aim of the presented study was to establish the degree of complexity of 5' NC region HCV genome and grouping of hepatitis C isolates by restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP) by the use of reverse - transcription - polymerase chain reaction products (RT-PCR). Representatives were checked by line probe assay. Isolated RNA preparations from whole blood or serum samples obtained from 55 patients with chronic HCV infection were used for cDNA synthesis. The nested polymerase chain reaction, RFLP experiments, SSCP analysis, the line probe assay and the phylogenetic analysis have shown that the applied methods RFLP and SSCP applied simultaneously to the analysis of the 5' NC virus region provided good evidence, that the region is highly complex. This phenomenon was examined by INNO-LIPA HCV II tests, used normally for genotyping, and it was shown, that the applied RFLP and SSCP methods alone are sufficient to describe the heterogeneity of this region.
Keywords: hepatitis C virus (HCV), Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP), genetic heterogeneit
Editorial
01 April 2025 : Editorial
Editorial: Rapid Testing for the Avian Influenza A(H5N1) Virus is Urgently Required as Infections in Poultry and Dairy Cows are on the Rise, and so is Transmission to HumansDOI: 10.12659/MSM.949109
Med Sci Monit 2025; 31:e949109
In Press
Clinical Research
Serum Prolidase and Ischemia-Modified Albumin Levels in Neural Tube Defects: A Comparative Study of Myelome...Med Sci Monit In Press; DOI: 10.12659/MSM.947873
Clinical Research
Impact of Depression, Fatigue, and Pain on Quality of Life in Slovak Multiple Sclerosis PatientsMed Sci Monit In Press; DOI: 10.12659/MSM.947630
Clinical Research
Longitudinal Evaluation of Metabolic Benefits of Inactivated COVID-19 Vaccination in Diabetic Patients in T...Med Sci Monit In Press; DOI: 10.12659/MSM.947450
Clinical Research
Impact of Voltage Level on Hospitalization and Mortality in Electrical Injury Cases: A Retrospective Analys...Med Sci Monit In Press; DOI: 10.12659/MSM.947675
Most Viewed Current Articles
17 Jan 2024 : Review article 8,000,435
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research 702,963
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Mar 2024 : Editorial 30,040
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...DOI :10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
28 Jan 2024 : Review article 23,978
A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and FutureDOI :10.12659/MSM.943912
Med Sci Monit 2024; 30:e943912