01 September 1997
In vitro evaluation of peritoneal glucose transport in the rabbit
Krystyna Czyżewska, Beata Szary, Jacek WaniewskiMed Sci Monit 1997; 3(5): BR637-641 :: ID: 501517
Abstract
To evaluate the rate of glucose diffusion through separate transport barriers against the net solute transport during peritoneal dialysis, a layer of mesothelium with adjacent connective tissue from rabbits was investigated in a modified Ussing chamber. The transport of glucose of initial feed concentrations 1.2, 2.0 and 3.6 g% (in Hanks' fluid) from the mesothelial to the interstitial side of the membrane was studied with the mesothelium intact or denuded (2.5 mmol/l sodium deoxycholate) peritoneum for 120 min or 150min, respectively. A mathematical model was used to calculate the diffusive permeability (P) for the investigated specimens. The model assumed diffusion transport between two well mixed compartments, and took into account the change of the concentration in the feed solution and the correction for sampling. The transport rates were stable across all studies with the intact peritoneum, and P was 2.62±1.18x10-4 cm/s (mean±SD). This P value used for a membrane area 1.75 m2, as assumed usually for CAPD patients, yields glucose clearance through the investigated barrier of 4.58 ml/s, which is higher than value reported from clinical transport studies. Removal of the mesothelium increased the transport coefficient (P) by about 11, 40% and 90% for the 1.2, 2.0 and 3.6 g% glucose concentration, respectively. We conclude that the mesothelium and adjacent connective tissue layer may be an important transport barrier.
Keywords: peritoneal transport, mesothelium, interstitium, Diffusion, Glucose
377 0
Editorial
01 May 2023 : Editorial
Editorial: Twenty Years On from Sequencing the Human Genome, Personalized/Precision Oncology Prepares to Meet the Challenges of Checkpoint Inhibitor TherapyDOI: 10.12659/MSM.940911
Med Sci Monit 2023; 29:e940911
In Press
28 May 2023 : Database Analysis
Scientometric and Visualized Analysis of Intravoxel Incoherent Motion Magnetic Resonance Imaging: 1988-2021Med Sci Monit In Press; DOI: 10.12659/MSM.938715
25 May 2023 : Database Analysis
The COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in PolandMed Sci Monit In Press; DOI: 10.12659/MSM.940904
25 May 2023 : Review article
A Review of Preclinical and Clinical Studies in Support of the Role of Non-Steroidal Anti-Inflammatory Drug...Med Sci Monit In Press; DOI: 10.12659/MSM.940635
25 May 2023 : Clinical Research
Periapical Lesions and Missed Canals in Endodontically Treated Teeth: A Cone-Beam Computed Tomographic Stud...Med Sci Monit In Press; DOI: 10.12659/MSM.940533
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952