01 September 1997
In vitro evaluation of peritoneal glucose transport in the rabbit
Krystyna Czyżewska, Beata Szary, Jacek WaniewskiMed Sci Monit 1997; 3(5): BR637-641 :: ID: 501517
Abstract
To evaluate the rate of glucose diffusion through separate transport barriers against the net solute transport during peritoneal dialysis, a layer of mesothelium with adjacent connective tissue from rabbits was investigated in a modified Ussing chamber. The transport of glucose of initial feed concentrations 1.2, 2.0 and 3.6 g% (in Hanks' fluid) from the mesothelial to the interstitial side of the membrane was studied with the mesothelium intact or denuded (2.5 mmol/l sodium deoxycholate) peritoneum for 120 min or 150min, respectively. A mathematical model was used to calculate the diffusive permeability (P) for the investigated specimens. The model assumed diffusion transport between two well mixed compartments, and took into account the change of the concentration in the feed solution and the correction for sampling. The transport rates were stable across all studies with the intact peritoneum, and P was 2.62±1.18x10-4 cm/s (mean±SD). This P value used for a membrane area 1.75 m2, as assumed usually for CAPD patients, yields glucose clearance through the investigated barrier of 4.58 ml/s, which is higher than value reported from clinical transport studies. Removal of the mesothelium increased the transport coefficient (P) by about 11, 40% and 90% for the 1.2, 2.0 and 3.6 g% glucose concentration, respectively. We conclude that the mesothelium and adjacent connective tissue layer may be an important transport barrier.
Keywords: peritoneal transport, mesothelium, interstitium, Diffusion, Glucose
Editorial
15 August 2022 : Editorial
Editorial: The Metabolic (Dysfunction) Associated Fatty Liver Disease (MAFLD)-Non-Alcoholic Fatty Liver Disease (NAFLD) Debate: A Forced Consensus and The Risk of a World DivideDOI: 10.12659/MSM.938080
Med Sci Monit 2022; 28:e938080
In Press
12 Aug 2022 : Clinical Research
Risk Factors and Pathogen Spectrum in Continuous Ambulatory Peritoneal Dialysis-Associated Peritonitis: A S...Med Sci Monit In Press; DOI: 10.12659/MSM.937112
11 Aug 2022 : Meta-Analysis
Optimal Surgical Treatment Method for Anterior Cruciate Ligament Rupture: Results from a Network Meta-AnalysisMed Sci Monit In Press; DOI: 10.12659/MSM.937118
10 Aug 2022 : Review article
Programmed Cell Death in Diabetic Nephropathy: A Review of Apoptosis, Autophagy, and NecroptosisMed Sci Monit In Press; DOI: 10.12659/MSM.937766
10 Aug 2022 : Database Analysis
Use and Effects of Augmentation of Labor with Oxytocin: A Single-Center, Retrospective, Case-Control Study ...Med Sci Monit In Press; DOI: 10.12659/MSM.937557
Most Viewed Current Articles
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Nov 2020 : Review article
Long-Term Respiratory and Neurological Sequelae of COVID-19DOI :10.12659/MSM.928996
Med Sci Monit 2020; 26:e928996