Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 November 1999

Increased apoptosis in freshly isolated lymphocytes from systemic lupus erythematosus patients

Dessislava Iv. Dimitrova, Anastassia P. Michailova, Boriana P. Deliyska, Elissaveta J. Naumova

Med Sci Monit 1999; 5(6): BR1049-1054 :: ID: 502962


Systemic lupus erythematosus (SLE) is an autoimmunological disease that is characterized by an abnormal immune response and autoantibody production. We investigated the apoptotic phenomenon in freshly isolated lymphocytes from lupus patients with different disease activities and compared the apoptosis of these cells with normal healthy controls. This was based on the suggested abnormalities in lymphocyte apoptosis (murine SLE model and human lupus) and reduction of the in vitro effects. In order to determine the immunosuppressive drug effect on lymphocyte apoptosis we looked for a correlation between immunosuppressive drug doses and percentage of apoptotic lymphocytes. We analysed isolated PBMC from 32 SLE patients and 17 controls for the presence of apoptosis by means of three methods (TUNNEL, Cell cycle analysis and LISA) detecting different points of the nuclear chromatin digestion pathway. The percentage of apoptotic lymphocytes was significantly higher in patients with active SLE, compared to the inactive disease (p < 0.001) and controls (p < 0.001). In the group of patients with inactive SLE the level of apoptotic cells was slightly higher (p=0.04), in comparison with healthy individuals. We observed no correlation (p=0.15) between immunosuppressive drug doses and the percentage of apoptotic lymphocytes. We established a significant positive correlation (r=0.55, p < 0.01) between the amount of nucleosomes in the cytoplasmic fraction of cell lysates and serum antinuclear antibody levels in active lupus. These findings support the hypothesis that apoptosis being a potential source for the circulating nucleosomal DNA identified from systemic lupus erythematosus patient plasma, may contribute to autoantibody responses.

Keywords: systemic lupus erythematosus, Nucleosomes

Add Comment 0 Comments


01 April 2024 : Editorial  

Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug Resistance

Dinah V. Parums

DOI: 10.12659/MSM.944600

Med Sci Monit 2024; 30:e944600


In Press

15 Apr 2024 : Laboratory Research  

The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in Osteoarthritis

Med Sci Monit In Press; DOI: 10.12659/MSM.943738  


07 Mar 2024 : Clinical Research  

Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...

Med Sci Monit In Press; DOI: 10.12659/MSM.943468  


08 Mar 2024 : Animal Research  

Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...

Med Sci Monit In Press; DOI: 10.12659/MSM.943443  


18 Apr 2024 : Clinical Research  

Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...

Med Sci Monit In Press; DOI: 10.12659/MSM.944127  


Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750