Logo Medical Science Monitor

Call: +1.631.470.9640
Closed: National Holiday

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 November 1999

Effect of glutamic acid, aspartic acid, glycine and gamma amino butyric acid on the growth of human leukemia and melanoma cells in vitro

Harrison Boldizas‡r, BŽela Szende, Ferenc Tima‡r

Med Sci Monit 1999; 5(6): BR1059-1064 :: ID: 502974

Abstract

K 562 human leukemia cells and HT 168 melanoma cells were cultured for 72 hours in Minimal Essential Medium (MEM) with or without asparagine. (AspNH2) and supplemented with 2.6 mmol/l glycine (Gly), 1.9 mmol/l gamma amino butyric acid (GABA), 1.5 mmol/l aspartic acid (Asp) or 6.8 mmol/l glutamic acid (Glu). Gly significantly inhibited the proliferation of both types of tumor cells both in the presence and the absence of AspNH2. This inhibitory effect developed gradually, usually by the 48th hour of incubation. Some of the Gly-treated cells showed signs indicative of apoptosis. In AspNH2-free MEM both the control and the experimental cell groups (supplemented with Gly, GABA, Asp or Glu) showed much slower proliferation than in MEM containing AspNH2. The values obtained in GABA-supplemented MEM in 72 hour experiment did not differ significantly from the control. Glu and Asp induced a significant rise in the growth of leukemia cells by the 3rd day of incubation. The growth of melanoma cells in Glu- or Asp-containing MEM did not significantly
differ from the control but significantly exceeded the value obtained in MEM supplemented with Gly. It is supposed that Cl-transport into tumor cells decreases and Na+ transport increases as a result of changes in the extracellular/intracellular ratio of excitatory/inhibitory amino acids. This results in depolarization which maintains cell division. The results suggest that some non-essential, transmitter amino acids exert either inhibitory or stimulating effect on tumor cell proliferation. This finding may open up new possibilities in preventing tumor growth.

Keywords: leukaemia and melanoma cells, transmitter amino acids, tumor cell proliferation

Add Comment 0 Comments

549 0

Editorial

01 May 2023 : Editorial  

Editorial: Twenty Years On from Sequencing the Human Genome, Personalized/Precision Oncology Prepares to Meet the Challenges of Checkpoint Inhibitor Therapy

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.940911

Med Sci Monit 2023; 29:e940911

In Press

28 May 2023 : Database Analysis  

Scientometric and Visualized Analysis of Intravoxel Incoherent Motion Magnetic Resonance Imaging: 1988-2021

Med Sci Monit In Press; DOI: 10.12659/MSM.938715  

25 May 2023 : Database Analysis  

The COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland

Med Sci Monit In Press; DOI: 10.12659/MSM.940904  

25 May 2023 : Review article  

A Review of Preclinical and Clinical Studies in Support of the Role of Non-Steroidal Anti-Inflammatory Drug...

Med Sci Monit In Press; DOI: 10.12659/MSM.940635  

25 May 2023 : Clinical Research  

Periapical Lesions and Missed Canals in Endodontically Treated Teeth: A Cone-Beam Computed Tomographic Stud...

Med Sci Monit In Press; DOI: 10.12659/MSM.940533  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750