Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 November 1999

Kupffer cell inhibition and its effect on ischemia/reperfusion injury as well as the biotransforming potential of the liver

Ryszard Wiaderkiewicz, Anna Wiaderkiewicz, Danuta Plewka, Marcin Kamiński, Artur Caban, Grzegorz Oczkowicz, Stanisław Kuśmierski

Med Sci Monit 1999; 5(6): BR1074-1080 :: ID: 503100

Abstract

Kupffer cells are believed to play an important role during ischemia-reperfusion (IR) injury of the liver, mostly by releasing pro-inflammatory factors and oxygen free radical formation. In our study we investigated the effect of pharmacological Kupffer cell inhibition by gadolinium chloride (GdCl3 Ð a selective Kupffer cell toxicant) on IR injury, as well as the biotransforming potential of the liver intoxicated with the organophosphorous insecticide Ð chlorfenvinphos. The study was performed on rats divided into 6 groups. Group 1 served as the control. Group 2 rats were subjected to 60 min of ischemia followed by 24 hours of reperfusion (I/R). Group 3 rats received chlorfenvinphos (i.p. Ð 2 mg/kg body weight) and were sacrificed after 24 hours. Group 4 rats received chlorfenvinphos after 30 min of ischemia and were sacrificed after 24 hours of reperfusion. Group 5 rats were subjected to I/R and GdCl3 was injected (i.v.) prior to reperfusion. Group 6 comprised rats subjected to I/R, treated with GdCl3 as mentioned above and injected with chlorfenvinphos. Liver sections were evaluated morphologically (H&E staining) and histochemically (SDH, LDH, G6Pase, Mg+2 ATPase and AcP activity). We evaluated the total content of cytochromes P450 and b5, activities of their corresponding NADPH and NADH reductases, as well as the presence of CYP1A1, CYP2E1 and CYP2B1/B2 isoforms in the microsomal fraction of the liver. I/R affects all studied enzyme activities and reduces the cytochrome P450 content and activity of its NADPH reductase. Amongst the studied isoforms, CYP2B1/B2 are mostly affected by IR. Intraperitoneal injection of chlorfenvinfos alone caused slight damage to the liver, although dramatically affected all studied parameters in rats subjected to I/R. The injection of gadolinium chloride alone has no effect on the structure nor biotransforming potential of the liver. However, the injection of GdCl3 prior to reperfusion demonstrated a beneficial effect on the activity of marker enzymes and diminished the morphological liver changes caused by I/R.

Keywords: P450 isoforms, Kupffer cells, gadolinium chloride, Reperfusion, Ischemia

Add Comment 0 Comments

Editorial

01 April 2024 : Editorial  

Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug Resistance

Dinah V. Parums

DOI: 10.12659/MSM.944600

Med Sci Monit 2024; 30:e944600

0:00

In Press

10 Apr 2024 : Clinical Research  

Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...

Med Sci Monit In Press; DOI: 10.12659/MSM.942612  

06 Mar 2024 : Clinical Research  

Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...

Med Sci Monit In Press; DOI: 10.12659/MSM.941102  

06 Mar 2024 : Clinical Research  

Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943884  

08 Mar 2024 : Clinical Research  

Evaluation of Foot Structure in Preschool Children Based on Body Mass

Med Sci Monit In Press; DOI: 10.12659/MSM.943765  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750