01 July 2008
Med Sci Monit 2008; 14(7): BR141-146 :: ID: 863660
Background: In a rat model, ghrelin has been shown to exert an anti-inflammatory effect in cardiovascular disease and arthritis. It also inhibits expression of proinflammatory cytokines. The wide tissue distribution of ghrelin expression and the presence of growth hormone secretagogue receptor (GHS-R) in the lung suggest that ghrelin may be a potential signal modulator in the lung. However, whether ghrelin exerts anti-inflammatory effects on acute lung injury induced by lipopolysaccharide (LPS) remains unknown. Therefore, we sought to investigate the role of ghrelin in LPS-induced acute lung injury and its underlying mechanism.
Material/Methods: We induced acute lung injury in rats via intratracheal instillation of LPS. We injected ghrelin and Nomega-nitro-L-arginine methyl ester (L-NAME) through the tail vein. Lung injury was assessed by histologic examination 6 hours after injury. Lung macrophages were isolated and incubated with LPS, L-NAME, and ghrelin. Concentrations of TNF-alpha and IL-1beta in bronchoalveolar lavage (BAL) fluid and culture supernatant were determined by enzyme-linked immunosorbent assay (ELISA). Nitric oxide (NO) in BAL fluid and culture supernatant and NO synthase (NOS) in cultured macrophage were detected by a spectrophotometry.
Results: Ghrelin attenuated pulmonary inflammation in LPS-induced acute lung injury, decreased production of proinflammatory cytokines, and increased NO concentrations in BAL fluid. Ghrelin also suppressed LPS-induced expression of proinflammatory cytokines, and increased NOS activity in cultured macrophages and NO concentrations in culture supernatants. The anti-inflammatory effect of ghrelin was inhibited by L-NAME.
Conclusions: Ghrelin attenuates LPS-induced acute lung inflammation and suppresses LPS-induced proinflammatory cytokine production in lung macrophages, which is partially mediated by increased NO production.
Keywords: Ghrelin, acute lung injury, Nitric Oxide, Bronchoalveolar Lavage Fluid, Culture Media, Cytokines - biosynthesis, Ghrelin - pharmacology, Inflammation Mediators - metabolism, Lipopolysaccharides - pharmacology, Macrophages, Alveolar - enzymology, Nitric Oxide - metabolism, Nitric Oxide Synthase - metabolism, Respiratory Distress Syndrome, Adult - prevention & control
01 August 2022 : EditorialEditorial: Long-Term Effects of Symptomatic and Asymptomatic SARS-CoV-2 Infection in Children and the Changing Pathogenesis of Common Childhood Viruses Driven by the COVID-19 Pandemic
Med Sci Monit 2022; 28:e937927
08 Aug 2022 : Clinical ResearchA 3-Year Clinical Evaluation of Endodontically Treated Posterior Teeth Restored with Resin Nanoceramic Comp...
Med Sci Monit In Press; DOI: 10.12659/MSM.937331
08 Aug 2022 : Clinical ResearchHemoglobin/Red Blood Cell Distribution Width Ratio in Peripheral Blood Is Positively Associated with Progno...
Med Sci Monit In Press; DOI: 10.12659/MSM.937146
05 Aug 2022 : Clinical ResearchA Retrospective Study of 98 Elderly Patients with High-Risk Lateral Femoral Wall Intertrochanteric Hip Frac...
Med Sci Monit In Press; DOI: 10.12659/MSM.936923
Most Viewed Current Articles
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292