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01 December 2008

Multiparameter flow cytometry characterization of MHC class I negative mouse bone marrow cells

Mattia QuartaABCDEFG, Deborah StrokaABCDE, Adrian KeoghABCDE, Daniel SidlerBCDEF, Itzhak AvitalABCDEF, Beat GloorADEG, Maurizio MuracaADEG, Daniel CandinasACDEFG, Daniel InderbitzinABCDEFG

Med Sci Monit 2008; 14(12): BR286-293 :: ID: 869477

Abstract

Background
MHC-I down-regulation was described in foetal liver progenitors, and two different subsets of adult bone marrow derived stem cells. These cells, namely, MHC-I-/Thy1+ bone marrow derived liver stem cells (BMDLSC) and the multipotent adult progenitors (MAPC) differentiated into functioning hepatocytes. The aim of this paper was to characterize the MHC-I negative bone marrow compartment as it pertains to BMDLSC and MAPC.
Material and Method
We performed multiparameter flow-cytometry analyses of the MHC-I negative compartment using hematopoietic (CD45, Ter119), and stem cell markers (Thy1.2, c-Kit, IL-3R, CD34) in adult mice.
Results
When analysing CD45 and Ter119 expression, the MHC-I negative bone marrow compartment divides into four sub-populations: 1. CD45-/Ter119+: 86.0+/-4.4%; 2. CD45+/Ter119+: 0.2+/-0.1%; 3. CD45+/Ter119-: 11.6+/-3.0%; 4. CD45-/Ter119-: 2.0+/-2.1%. Stem cells markers were only expressed on MHC-I negative/ CD45+/Ter119- cells. In vivo, MAPC (Ter119-/CD45- cells) are composed of MHC-I negative (24%) and MHC-I positive cells and do not express any of the stem cell markers tested.
Conclusions
In conclusion, mouse BMDLSC and MAPC are two distinct stem cell populations. Down-regulation of MHC-I was the only common characteristic found between BMDLSC and MAPC suggesting that selection of MHC-I negative cells might represent an efficient strategy to enrich for bone marrow stem cells with liver developmental potential.

Keywords: Mice, Inbred C57BL, beta 2-Microglobulin - metabolism, Mice, Inbred BALB C, Flow Cytometry - methods, Histocompatibility Antigens Class I - analysis, Bone Marrow Cells - metabolism, Blood Group Antigens - analysis, Antigens, CD45 - metabolism

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750