29 January 2009
The hemicellulose preparation, Natramune (PDS-2865), increases macrophage phagocytosis and nitric oxide production and increases circulating human lymphocytes levels
Benjamin S. WeeksACDEFG, Pedro P. PerezABEFMed Sci Monit 2009; 15(2): BR43-46 :: ID: 869549
Abstract
Background
Hemicellulose containing nutritional supplements demonstrate benefits to the immune system in vitro and in vivo. Here we show that Natramune (PDS-2865) stimulates phagocytosis, nitric oxide production and boosts viability in J774A.1 murine machrophages. We also show that dietary supplementation with Natramune (PDS-2865) significantly increases the levels of circulating lymphocytes in human subjects.
Material and Method
In order to measure the beneficial effects of Natramune (PDS-2865) on cells of the immune system, phagocytosis was measured by J774A.1 uptake of fluorescently labeled E. Coli bioparticles, nitric oxide production was measured by the formation of nitrite, and cell proliferation and viability was measured by NADH reduction of WST-8. The effect of Natramune (PDS-2865) on human circulating leukocyte levels was measured in 18 volunteers after an 8 week regimen of two 250 mg doses daily after which blood was collected and blood cell number and types were counted.
Results
Natramune (PDS-2865) stimulated phagocytosis, nitric oxide production and promoted proliferation/viability in J774A.1 cells by 65%, 517%, and 155% respectively. Further, Natramune (PDS-2865) did boost human circulating total lymphocyte levels (18%) in a statistically significant manner and while all lymphocyte subtype levels also increased, the individual subtype increases were not statistically significant.
Conclusions
Dietary supplementation with Natramune (PDS-2865) enhances immune system function and vitality.
Keywords: Polysaccharides - pharmacology, Phagocytosis - drug effects, Nitric Oxide - biosynthesis, Macrophages - immunology, Lymphocytes - immunology, Immune System - drug effects, Cell Survival - drug effects, Cell Proliferation - drug effects, Cell Movement - drug effects, Cell Count, Adolescent
Editorial
01 August 2022 : Editorial
Editorial: Long-Term Effects of Symptomatic and Asymptomatic SARS-CoV-2 Infection in Children and the Changing Pathogenesis of Common Childhood Viruses Driven by the COVID-19 PandemicDOI: 10.12659/MSM.937927
Med Sci Monit 2022; 28:e937927
In Press
10 Aug 2022 : Review article
Programmed Cell Death in Diabetic Nephropathy: A Review of Apoptosis, Autophagy, and NecroptosisMed Sci Monit In Press; DOI: 10.12659/MSM.937766
10 Aug 2022 : Database Analysis
Use and Effects of Augmentation of Labor with Oxytocin: A Single-Center, Retrospective, Case-Control Study ...Med Sci Monit In Press; DOI: 10.12659/MSM.937557
08 Aug 2022 : Clinical Research
A 3-Year Clinical Evaluation of Endodontically Treated Posterior Teeth Restored with Resin Nanoceramic Comp...Med Sci Monit In Press; DOI: 10.12659/MSM.937331
08 Aug 2022 : Clinical Research
Hemoglobin/Red Blood Cell Distribution Width Ratio in Peripheral Blood Is Positively Associated with Progno...Med Sci Monit In Press; DOI: 10.12659/MSM.937146
Most Viewed Current Articles
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Nov 2020 : Review article
Long-Term Respiratory and Neurological Sequelae of COVID-19DOI :10.12659/MSM.928996
Med Sci Monit 2020; 26:e928996