Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

21 December 2009

Smoking and other personal characteristics as potential predictors for fecal bacteria populations in humans

Ikuko KatoABCDEFG, Jordan M NechvatalBEF, Sijana DzinicBEF, Marc D BassonADEG, Adhip P MajumdarADEG, Jeffrey L Ram

Med Sci Monit 2010; 16(1): CR1-7 :: ID: 878298


Intestinal microbes have been postulated to play an important role in the development of colorectal cancer. Recently developed methods for preserving and delivering fecal samples at ambient temperature to the laboratory for molecular analysis of bacterial constituents were used to test associations of bacterial populations with epidemiologic risk factors for colorectal cancer.
Material and Method
Real-time PCR targeting 16S rRNA gene sequences was used to quantify three intestinal bacterial groups relative to total DNA in stool samples preserved with RNAlater from 62 subjects. Subjects' medical and family history, race, diet, weight, height, and personal habits including smoking were obtained through structured questionnaires.
Bacteroides DNA proportions were relatively stable among individuals and relatively independent of dietary intake or other personal factors. Clostridium (coccoides group) DNA was positively associated with total fat and vitamin C intake. Desulfovibrio DNA amount tended to be higher in African Americans than in other races. Furthermore, Desulfovibrio DNA increased progressively with pack-years of cigarette smoking. The relative DNA quantity (%) was more than 17 times higher in the subjects who smoked at least 15 pack-years compared with never-smokers (P-value for a linear trend =0.001). In addition, Desulfovibrio DNA (%) decreased with increased calcium, vitamin E, and dietary fiber intake. However, only smoking remained significant in multivariable analysis.
Although the study was limited by its sample size, these results suggest that smoking (or possibly unmeasured dietary confounders) may exert modulatory effects on the bacterial populations of the gastrointestinal tract. The study also demonstrates collection, preservation, and sample delivery procedures suitable for large epidemiological studies.

Keywords: Questionnaires, RNA, Ribosomal, 16S - genetics, Polymerase Chain Reaction, Multivariate Analysis, Michigan, Diet, Feces - microbiology, Desulfovibrio - genetics, Colorectal Neoplasms - microbiology, Clostridium - genetics, Body Mass Index, Bacteroides - genetics, African Americans, Smoking - adverse effects

Add Comment 0 Comments


01 February 2024 : Editorial  

Editorial: Global Health Concerns as Vaccine-Preventable Infections Including SARS-CoV-2 (JN.1), Influenza, Respiratory Syncytial Virus (RSV), and Measles Continue to Rise

Dinah V. Parums

DOI: 10.12659/MSM.943911

Med Sci Monit 2024; 30:e943911


In Press

02 Jan 2024 : Clinical Research  

Urinary Concentration of Renal Biomarkers in Healthy Term Neonates: Gender Differences in GST-pi Excretion

Med Sci Monit In Press; DOI: 10.12659/MSM.942819  


29 Dec 2023 : Clinical Research  

Performance of Subcutaneous Implantable Cardioverter Defibrillator (S-ICD) in Chinese Population with Prima...

Med Sci Monit In Press; DOI: 10.12659/MSM.942747  

08 Jan 2024 : Review article  

Review of Approaches to the Management of Vertical Dental Root Fractures and Tooth Preservation

Med Sci Monit In Press; DOI: 10.12659/MSM.943100  

03 Jan 2024 : Clinical Research  

Comparative Efficacy of Ultrasound-Guided Cervical Fascial Infiltration versus Periarticular Administration...

Med Sci Monit In Press; DOI: 10.12659/MSM.942044  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750