Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

21 December 2009

The reliability of ELISA in predicting H. pylori infection in dyspeptic populations under age 45

Malgorzata PalkaABCDEF, Tomasz TomasikACDG, Adam WindakACDG, Grzegorz MargasABCDE, Tomasz MachACDEG, Agnieszka BohonosEF

Med Sci Monit 2010; 16(1): PH24-28 :: ID: 878313


Dyspepsia is a common problem worldwide. Many dyspeptic patients have no alarm symptoms and are diagnosed by family doctors. Non-invasive testing of H. pylori is recommended in patients up to the age of 55. Testing of asymptomatic high risk groups is now being considered in many countries. The aim of the study was to compare the accuracy of serology tests (ELISA, HelicoTest) against the benchmark UBT (urea breath test) in the detection of H. pylori infection in young populations.
Material and Method
The study was conducted on 238 patients under age 45 (159 with undiagnosed dyspepsia and 79 controls). The gold standard of diagnosis of H. pylori infection was based on a positive UBT. UBT, serological ELISA and HelicoTest were performed on each of the patients.
By UBT 50.9% and by ELISA 49.7% of dyspeptic patients were infected with H. pylori, while in the control group 38% and 39.2% were infected, respectively. HelicoTest showed the highest positive results in both groups (55.3% study group, 44.3% control). The average level of IgG antibodies from ELISA testing among patients with a positive UBT H. pylori result was 3.16 U/ml. Patients with negative UBT results showed an average IgG level of 0.53 U/ml.
There was a correlation of increasing antibody level on ELISA and probability of testing positive on UBT. The majority of patients had moderate dyspeptic symptoms. The ELISA is an easy, cheap and effective method for diagnosing H. pylori infection in the primary care setting.

Keywords: Immunoglobulin G - analysis, Helicobacter Infections - diagnosis, Helicobacter pylori, Enzyme-Linked Immunosorbent Assay - statistics & numerical data, Dyspepsia - microbiology, Breath Tests - methods, Poland



01 February 2023 : Editorial  

Editorial: The XBB.1.5 (‘Kraken’) Subvariant of Omicron SARS-CoV-2 and its Rapid Global Spread

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939580

Med Sci Monit 2023; 29:e939580


19 January 2023 : Clinical Research  

Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...

Med Sci Monit In Press; DOI: 10.12659/MSM.938892  

27 December 2022 : Clinical Research  

Effect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...

Med Sci Monit 2022; 28:e938926

10 December 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit 2022; 28:e938647

In Press

03 Feb 2023 : Clinical Research  

Treatment of Gingival Recession Defects with Pouch and Tunnel Technique Using Connective Tissue Graft and L...

Med Sci Monit In Press; DOI: 10.12659/MSM.938865  

02 Feb 2023 : Clinical Research  

Online Questionnaire-Based Study to Evaluate the Attitudes and Use of Rubber Dental Dams by Saudi Dental Pr...

Med Sci Monit In Press; DOI: 10.12659/MSM.938672  

02 Feb 2023 : Database Analysis  

Prognostic-Related Biomarkers in Pancreatic Ductal Adenocarcinoma Correlating with Immune Infiltrates Based...

Med Sci Monit In Press; DOI: 10.12659/MSM.938785  

01 Feb 2023 : Clinical Research  

Comparison of 3 Enzyme-Linked Immunoassay Methods to Evaluate Serum Concentrations of Infliximab and Antibo...

Med Sci Monit In Press; DOI: 10.12659/MSM.939084  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750