Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 February 2010

Higher expression patterns of the intestinal stem cell markers Musashi-1 and hairy and enhancer of split 1 and their correspondence with proliferation patterns in the mouse jejunum

Tao YuABE, Qi-Kui ChenADG, Yu GongBC, Zhong-Sheng XiaEF, Crista R. RoyalCF, Kai-Hong HuangE

Med Sci Monit 2010; 16(2): BR68-74 :: ID: 878355


Stem cells must be located and positively identified to elucidate their role in disease pathogenesis and therapy. In this study the expression patterns of the small intestinal stem cell (SISC) markers Musashi-1 (Msi1) and hairy and enhancer of split 1 (Hes1) were identified and the relationship between their expression and epithelial proliferation in the whole mouse small intestine was examined.
Material and Method
Mouse small intestines were separated into four segments. Msi1 and Hes1 expression levels were quantified in each intestinal segment by immunohistochemistry, real-time reverse-transcription polymerase chain reaction, and Western blot. Small intestinal epithelial proliferation was examined using the bromodeoxyuridine (BrdU) method and proliferating cell nuclear antigen (PCNA) immunostaining.
Msi1- and Hes1-positive cells were predominantly detected at the crypt bases. Msi1 and Hes1 protein expression values were significantly higher in the jejunal segment than in the ileal (P<0.05) and mRNA was directly associated with protein expression values. Greater numbers of proliferative cells were detected in jejunal crypts with BrdU and PCNA labeling (P<0.05).
Expression of the Msi1 and Hes1 SISC markers in the small intestine was not homogenous and the markers were more highly expressed in jejunal tissues. This expression pattern correlated with the small intestinal epithelial proliferation pattern measured by BrdU- and PCNA-labeling methods.

Keywords: RNA-Binding Proteins - metabolism, RNA, Messenger - metabolism, Microvilli - metabolism, Nerve Tissue Proteins - metabolism, Jejunum - metabolism, Homeodomain Proteins - metabolism, Epithelial Cells - metabolism, Gene Expression Regulation, Biological Markers - metabolism, Basic Helix-Loop-Helix Transcription Factors - metabolism, Stem Cells - metabolism

Add Comment 0 Comments


01 April 2024 : Editorial  

Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug Resistance

Dinah V. Parums

DOI: 10.12659/MSM.944600

Med Sci Monit 2024; 30:e944600


In Press

05 Mar 2024 : Clinical Research  

Role of Critical Shoulder Angle in Degenerative Type Rotator Cuff Tears: A Turkish Cohort Study

Med Sci Monit In Press; DOI: 10.12659/MSM.943703  


06 Mar 2024 : Clinical Research  

Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943797  

21 Mar 2024 : Meta-Analysis  

Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...

Med Sci Monit In Press; DOI: 10.12659/MSM.943863  

10 Apr 2024 : Clinical Research  

Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...

Med Sci Monit In Press; DOI: 10.12659/MSM.942612  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750