01 October 2010
Peroxiredoxin1: A potential obesity-related factor in the hypothalamusQi-Ming WangABCE, Ying CaiABCE, De-Run TianAG, Hui YangDF, Zhong-Nan WeiCD, Fei WangBF, Ji-Sheng HanDF
Med Sci Monit 2010; 16(10): BR321-326 :: ID: 881190
Background: Obesity has become a global epidemic and contributes to the increasing burden of type 2 diabetes, cardiovascular disease and some cancers worldwide. However, the precise molecular mechanisms of obesity remain poorly elucidated. Recent data indicate monitoring of brain energy status especially in the hypothalamus, is crucial for energy homeostasis. Anti-oxidant defenses are suggested to play an important role in production of a well-balanced signal, which is necessary for adjusted neuronal activities.
Material/Methods: Hypothalamus proteomes of the diet-induced obesity (DIO) and diet-resistant (DR) rats were analyzed using 2-dimensional gel electrophoresis (2-DE) combined with MALDI-TOF/TOF-MS analysis.
Results: The 2-DE maps showed a resolution of 1280±15, 1250±10, 1260±12 protein spots in normal, DIO and DR groups, respectively. Twenty-two proteins were identified in the hypothalamus of DIO or DR rats. These proteins are involved in metabolism enzymes, antioxidant proteins, proteasome-related proteins and signaling proteins. In comparison with normal-diet group, peroxiredoxin1 (Prx1) was significantly decreased in DR group and it was increased in DIO group. The expression of Prx1 was further validated by Western blot analysis.
Conclusions: The differential expression of Prx1 in DR and DIO groups indicated that the antioxidant protein may play an important role in the genesis of DR or DIO. This study provides new clues to illuminate the mechanisms involved in the progress of DIO and DR in the hypothalamus.
Keywords: Obesity - metabolism, Proteins - metabolism, Motor Activity - physiology, Hypothalamus - metabolism, Homeodomain Proteins - metabolism, Diet, Random Allocation
01 June 2023 : EditorialEditorial: Infectious Disease Surveillance Using Artificial Intelligence (AI) and its Role in Epidemic and Pandemic Preparedness
Med Sci Monit 2023; 29:e941209
05 Jun 2023 : Clinical ResearchComparison of Texture and Color Enhancement Imaging with White Light Imaging in 52 Patients with Short-Segm...
Med Sci Monit In Press; DOI: 10.12659/MSM.940249
05 Jun 2023 : Laboratory ResearchComparison of Composite Resin (Duo-Shade) Shade Guide with Vita Ceramic Shades Before and After Chemical an...
Med Sci Monit In Press; DOI: 10.12659/MSM.940949
02 Jun 2023 : Database AnalysisThe COVID-19 Crisis and the Incidence of Alcohol-Related Deaths in Poland
Med Sci Monit In Press; DOI: 10.12659/MSM.940904
01 Jun 2023 : Clinical ResearchRoot Canal Numbers and Configurations in 1080 Permanent Canine Teeth in 270 Saudi Subjects Using Cone-Beam ...
Med Sci Monit In Press; DOI: 10.12659/MSM.940472
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : EditorialEditorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...
Med Sci Monit 2022; 28:e935952