Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 November 2010

Comparative molecular epidemiology of Leishmania major and Leishmania tropica by PCR-RFLP technique in hyper endemic cities of Isfahan and Bam, Iran

Monir DoudiAB, Seyed Hossein HejaziCG, Mohammad Reza RazaviD, Manizheh NarimaniE, Somayeh KhanjaniE, Gilda EslamiF

Med Sci Monit 2010; 16(11): CR530-535 :: ID: 881217


Background: Leishmania is an obligate intracellular protozoa, and the sandfly, as a vector, transmits infectious forms of the parasite to the vertebrate host. The etiologic agents of cutaneous leishmaniasis (CL), Leishmania major and Leishmania tropica, are the most prevalent factor in Iran, especially in the Isfahan and Bam regions. Because of the importance of CL in endemic regions and the interaction of species diversity factors in developing control strategies, several isolated Leishmania species from 2 hyperendemic regions of Iran, Isfahan and Bam cities, were examined in this study by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP).
Material/Methods: In this study, 340 samples were taken from clinically suspected CL patients to prepare slides for direct microscopy and cultures for promastigotes by PCR-RFLP. The internal transcribed spacer 1 (ITS1) region of genomic DNA was extracted and amplified with LITSr and L5.8s primers. Amplification by PCR-RFLP was performed to determine 4 kinds of genotype pattern of the species in the 2 main cities of Isfahan and Bam. Some of the product samples were sequenced and analyzed.
Results: Two genotypic groups were detected from L. major isolates, LmA and LmB; also L. tropica showed 2 patterns, LtA and LtB, in comparison with standard species. The most prevalent genotypes related to isolates of Isfahan were LmA and of Bam were LtA. These 2 genotypes were recorded as major etiologic factors of CL in these 2 regions.
Conclusions: Leishmania major and L. tropica, the causative agents of zoonotic CL and anthroponotic CL, respectively, in Isfahan and Bam, are genetically highly polymorphic species, and a correlation may exist between genetic heterogeneous and clinical manifestation and geographic regions of the disease in humans.

Keywords: Molecular Epidemiology - methods, Leishmaniasis, Cutaneous - epidemiology, Leishmania tropica - isolation & purification, Leishmania major - isolation & purification, Iran - epidemiology, Insect Vectors, Genotype, Polymerase Chain Reaction - methods, Polymorphism, Restriction Fragment Length, Psychodidae - parasitology

Add Comment 0 Comments

In Press

08 Mar 2024 : Clinical Research  

Evaluation of Foot Structure in Preschool Children Based on Body Mass

Med Sci Monit In Press; DOI: 10.12659/MSM.943765  


15 Apr 2024 : Laboratory Research  

The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in Osteoarthritis

Med Sci Monit In Press; DOI: 10.12659/MSM.943738  


07 Mar 2024 : Clinical Research  

Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...

Med Sci Monit In Press; DOI: 10.12659/MSM.943468  

08 Mar 2024 : Animal Research  

Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...

Med Sci Monit In Press; DOI: 10.12659/MSM.943443  

Most Viewed Current Articles

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799


14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990


16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387


01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750