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16 December 2010

The effect of L-ascorbic acid and/or tocopherol supplementation on electrophysiological parameters of the colon of rats chronically exposed to lead

Danuta I. Kosik-BogackaBDE, Irena Baranowska-BosiackaABCDE, Mariola MarchlewiczBD, Agnieszka KolasaBD, Katarzyna JakubowskaB, Maria OlszewskaB, Natalia LanochaB, Ireneusz WiernickiEF, Barbara MilloB, Barbara WiszniewskaG, Dariusz ChlubekG

DOI: 10.12659/MSM.881323

Med Sci Monit 2011; 17(1): BR16-26

Abstract

Background: The aim of this study was to assess the effect of diet supplementation with L-ascorbic acid (500 mg/L), tocopherol (3 mg/kg b.w.), and/or a water soluble analog of tocopherol (Trolox) (48 mg/L) on ion transport in the colon of rats subjected to a chronic exposure (9 months) to 0.1% lead acetate in drinking water.
Material/Methods: The electrophysiological parameters of the colon wall were measured with Ussing methods. Lead content in the whole blood was analyzed by graphite furnace atomic absorption spectrometry (GFAAS) using Zeeman correction. L-ascorbic acid and tocopherol in plasma was measured by high performance liquid chromatography. Immunohistochemical reaction was carried out for visualization of occludin, the intracellular tight junction protein.
Results: We showed a strong inhibitory effect of lead on the electrophysiological parameters, changes in intestinal permeability, disappearance of junctional occludin, decreased amount of mucus covering the colon surface, and the accumulation of PAS-positive substance in the apical region of the cytoplasm in the absorptive cells.
Conclusions: Supplementation with tocopherol or Trolox did not exert a beneficial influence on the studied parameters. L-ascorbic acid positively influenced the examined electrophysiological parameters, as it cancelled the inhibitory influence of lead on ion transport in the rat colon. L-ascorbic acid also protected against tight junction disruption of epithelial cells in the colon of the lead-treated rats. A similar effect was observed in the group of rats receiving lead and supplemented with L-ascorbic acid plus Trolox.

Keywords: Organometallic Compounds - toxicity, Occludin, Membrane Proteins - metabolism, Ion Transport - drug effects, Electrophysiology, Dietary Supplements, Colon - physiology, Chromatography, High Pressure Liquid, Ascorbic Acid - pharmacology, Tocopherols - pharmacology, Water Pollutants, Chemical - toxicity

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750