08 May 2014
Pressing the nerve alters muscle fiber types of the peroneus longus in rats: Preliminary evidence for external anal sphincteroplasty
Song ShiACEF, Hao LiuADG, Xiaobin BaiBF, Yongxiao CaoADDOI: 10.12659/MSM.884037
Med Sci Monit 2014; 20:752-757
Abstract
BACKGROUND: Studies have demonstrated that anal reconstruction with a gracilis graft pressing the dominant nerve could be used to treat fecal incontinence. However, the detailed mechanism by this remains unknown. Herein, we evaluated the alteration in muscle fiber types and contractility of the peroneus longus muscle in rats after pressing its dominant nerves.
MATERIAL AND METHODS: The rat soleus and peroneus longus were exposed during surgery. The superficial peroneal nerve was pressed so that the peroneus longus temporarily lost its innervation. The epimysium between the soleus and the peroneus longus was removed. The end point of the soleus was cut off and the epimysium of the contact surfaces of the soleus and the peroneus longus were sutured. Five months later, peroneus longus contractility was recorded by the myograph system, and types of muscle fibers were observed using the myosin ATPase staining method.
RESULTS: The skeletal muscle fiber type underwent adaptive changes due to double innervations with both fast and slow muscle nerves. Compared with other groups, the percentage of type I fibers in the peroneus longus increased significantly in the group of rats with the pressure on the nerve and removal of the sarcolemma. The maximal contraction and relaxation time at the single twitch and complete tetanus of the peroneus longus were also increased.
CONCLUSIONS: Our results show that pressing dominant nerves alter the skeletal muscle fiber types of the peroneus longus, which lead to increased maximal contraction and relaxation time, and significantly improve the ability in resistance to fatigue in rats. This study provides a basis for future clinical studies for external anal sphincter reconstruction using gracilis grafts that are doubly innervated by pressing on its dominant nerve.
Keywords: Anal Canal - surgery, Models, Biological, Muscle Contraction, Muscle Fibers, Skeletal - pathology, Nerve Crush, Pressure, Sciatic Nerve - physiopathology
Editorial
01 October 2024 : Editorial
Editorial: Potentials and Pitfalls in Targeting Glucagon-Like Peptide-1 (GLP-1) in the Management of Increasing Levels of ObesityDOI: 10.12659/MSM.946675
Med Sci Monit 2024; 30:e946675
In Press
Clinical Research
Anterior Disc Displacement and Cortication Patterns in the Temporomandibular JointMed Sci Monit In Press; DOI: 10.12659/MSM.945967
Review article
Lamotrigine: A Safe and Effective Mood Stabilizer for Bipolar Disorder in Reproductive-Age AdultsMed Sci Monit In Press; DOI: 10.12659/MSM.945464
Clinical Research
Impact of Manual Sustained Inflation vs Stepwise PEEP on Pulmonary and Cerebral Outcomes in Carotid Endarte...Med Sci Monit In Press; DOI: 10.12659/MSM.944936
Clinical Research
Predicting Vaginal Delivery Success: Role of Intrapartum Transperineal Ultrasound Angle of Descent at a Sin...Med Sci Monit In Press; DOI: 10.12659/MSM.945458
Most Viewed Current Articles
17 Jan 2024 : Review article 6,056,505
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research 1,846,289
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research 693,590
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
07 Jan 2022 : Meta-Analysis 257,892
Efficacy and Safety of Light Therapy as a Home Treatment for Motor and Non-Motor Symptoms of Parkinson Dise...DOI :10.12659/MSM.935074
Med Sci Monit 2022; 28:e935074