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22 March 2015 : Animal Research  

Proliferation and Differentiation of Rat Osteoporosis Mesenchymal Stem Cells (MSCs) after Telomerase Reverse Transcriptase (TERT) Transfection

Chao LiABCDEF, Guojun WeiABCDE, Qun GuABCDE, Qiang WangAB, Shuqin TaoABC, Liang XuABCDEFG

DOI: 10.12659/MSM.893144

Med Sci Monit 2015; 21:845-854

Abstract

BACKGROUND: The aim of this study was to determine whether MSC are excellent materials for MSCs transplantation in the treatment of osteoporosis.

MATERIAL AND METHODS: We studied normal, osteoporosis, and TERT-transfected MSC from normal and osteoporosis rats to compare the proliferation and osteogenic differentiation using RT-PCR and Western blot by constructing an ovariectomized rat model of osteoporosis (OVX). The primary MSC from model rats were extracted and cultured to evaluate the proliferation and differentiation characteristics.

RESULTS: MSCs of osteoporosis rats obviously decreased in proliferation ability and osteogenic differentiation compared to that of normal rats. In contrast, in TERT-transfected MSC, the proliferation and differentiation ability, and especially the ability of osteogenic differentiation, were significantly higher than in osteoporosis MSC.

CONCLUSIONS: TERT-transfected MSCs can help osteoporosis patients in whom MSC proliferation and osteogenic differentiation ability are weak, with an increase in both bone mass and bone density, becoming an effective material for autologous transplantation of MSCs in further treatment of osteoporosis. However, studies are still needed to prove the in vivo effect, biological safety, and molecular mechanism of TERT-osteoporosis treatment. Additionally, because the results are from an animal model, more research is needed in generalizing rat model findings to human osteoporosis patients.

Keywords: Blotting, Western, Antigens, CD - metabolism, Femur - pathology, Gene Expression Regulation, Genetic Vectors - metabolism, Lentivirus - metabolism, Mesenchymal Stromal Cells - pathology, Osteoporosis - pathology, Ovariectomy, Plasmids - metabolism, Real-Time Polymerase Chain Reaction, Telomerase - metabolism

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750