17 May 2016 : Clinical Research
Detection of Circulating Tumor Cells by Fluorescent Immunohistochemistry in Patients with Esophageal Squamous Cell Carcinoma: Potential Clinical ApplicationsShu-Ping LiABCEF, Quan-lin GuanAEG, Da ZhaoACEF, Guang-Jun PeiBCF, Hong-Xin SuBCF, Lan-Ning DuBC, Jin-Xiang HeBD, Zhao-Chen LiuDF
Med Sci Monit 2016; 22:1654-1662
BACKGROUND: Circulating tumor cells (CTCs) are tumor cells that leave the primary tumor site and enter the bloodstream, where they can spread to other organs; they are very important in the diagnosis, treatment, and prognosis of malignant tumors. However, few studies have investigated CTCs in esophageal squamous cell carcinoma (ESCC). The aim of this study was to investigate the CTCs in blood of ESCC patients and its potential relevance to clinicopathological features and prognosis.
MATERIAL AND METHODS: CTCs were acquired by a negative enrichment method that used magnetic activated cell sorting (MACSTM). Fluorescent immunohistochemistry (IHC) was used to identify the CTCs. Then, the positive CTC patients with ESCC were analyzed, after which the relationship between CTCs and clinicopathologic features was evaluated.
RESULTS: In the present study, 62 out of 140 (44.3%) patients with ESCC were positive for CTCs. The positive rate of CTCs was significantly related with stage of ESCC patients (P=0.013). However, there was no relationship between CTC status and age, sex, smoking tumor history, tumor location, differentiation of tumor, lymphatic invasion, or lymph venous invasion (P>0.05). Kaplan-Meier analysis showed that patients positive for CTCs had significantly shorter survival time than patients negative for CTCs. Multivariate analysis demonstrated that stage and CTC status were significant prognostic factors for patients with ESCC.
CONCLUSIONS: CTCs positivity is an independent prognostic biomarker that indicates a worse prognosis for patients with ESCC.
Keywords: Biomarkers, Tumor - blood, Carcinoma, Squamous Cell - pathology, Esophageal Neoplasms - pathology, Fluorescent Antibody Technique - methods, Immunohistochemistry - methods, Kaplan-Meier Estimate, Neoplastic Cells, Circulating - pathology, Prognosis
01 September 2023 : EditorialEditorial: A Rapid Global Increase in COVID-19 is Due to the Emergence of the EG.5 (Eris) Subvariant of Omicron SARS-CoV-2
Med Sci Monit 2023; 29:e942244
21 Sep 2023 : Clinical ResearchThe Impact of Implementing the Vortex Approach on Airway Management Performance in Stressed Medical Student...
Med Sci Monit In Press; DOI: 10.12659/MSM.940372
20 Sep 2023 : Clinical ResearchA Retrospective Study to Compare Pharyngeal Anatomical Changes Measured Before and After Treatment for Malo...
Med Sci Monit In Press; DOI: 10.12659/MSM.941749
20 Sep 2023 : Database AnalysisComparative Finite Element Analysis of Endocrowns and Traditional Restorations for Endodontically Treated M...
Med Sci Monit In Press; DOI: 10.12659/MSM.941314
Most Viewed Current Articles
13 Nov 2021 : Clinical ResearchAcceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292