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04 October 2016 : Laboratory Research  

Golgi Phosphoprotein 3 Inhibits the Apoptosis of Human Glioma Cells in Part by Downregulating N-myc Downstream Regulated Gene 1

Xin LiBE, Mengyou LiBF, Xiuli TianCD, Qingzhe LiBC, Qingyang LuBD, Qingbin JiaDF, Lianqun ZhangDF, Jinqiang YanBD, Xueyuan LiAG, Xingang LiAG

DOI: 10.12659/MSM.900349

Med Sci Monit 2016; 22:3535-3543

Abstract

BACKGROUND: Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in the development of several human cancers. Our previous study showed that GOLPH3 expression in glioma tissues was related to the severity of the malignancy of the cancer. However, the mechanism by which GOLPH3 affects cell apoptosis is largely unknown. The present study was designed to explore the possible mechanism of GOLPH3 in cell apoptosis.

MATERIAL AND METHODS: To analyze the biological role of GOLPH3 in glioma cells, we used GOLPH3 small interference RNA in apoptosis of glioma cells. The apoptosis of glioma cells was detected by flow cytometry. The expression level of GOLPH3 and NDRG1 protein was determined by Western blot analyses and immunohistochemical staining, respectively, to evaluate their association with glioma. Tumor tissues were collected from patients with glioma. Normal cerebral tissues were acquired from cerebral trauma patients undergoing internal decompression surgery.

RESULTS: We confirm that the decrease of GOLPH3 that promotes the apoptosis of glioma cells may be regulated by the activation of NDRG1 and cleaved capcase 3. There was a inverse association between GOLPH3 and NDRG1 in glioma samples.

CONCLUSIONS: Our findings indicate that GOLPH3 and NDRG1 both play an important role in glioma etiology. Either GOLPH3 or NDRG1 might be a potential candidate for malignant glioma therapy.

Keywords: Glioma, Golgi Apparatus

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750