Abstract

BACKGROUND: This case-control study aimed to analyze the association of XRCC2 polymorphisms (rs3218408 and rs3218384) with colorectal cancer (CRC) risk. The interaction of XRCC2 polymorphisms with environmental factors was investigated as well.

MATERIAL AND METHODS: We enrolled 147 CRC patients and 114 healthy individuals into the study. Polymerase chain reaction (PCR)-sequencing method was performed to detect rs3218408 and rs3218384 polymorphisms. Hardy-Weinberg equilibrium (HWE) was checked in the control group. Odds ratio (OR) with 95% confidence interval (CI) represented the risk of CRC. Cross-table method was used in analyzing the interaction effects.

RESULTS: Compared to the control group, the frequency of smokers was much higher in the case group (P<0.001). A similar result was observed in drinkers (55.8% vs. 40.4%, P=0.013). Dietary habits of all subjects were investigated as well, showing that CRC patients ate fewer vegetables than did healthy controls (P<0.001). In the analysis of polymorphisms, rs3218408 appeared to be an independent risk factor of CRC (GG: OR=2.048, 95%CI=1.032-4.061; G allele: OR=1.445, 95%CI=1.019–2.049). There were 68 (76.4%) C allele carriers (rs3218384) among smokers, which was higher than the number of G allele carriers (P<0.001). A similar outcome was observed for alcohol drinkers (P=0.048), which suggests a relationship of rs3218384 with smoking and drinking. Further analysis indicated that interaction of rs3218384 with smoking increased the risk of CRC (GG and smoking: OR=3.250, 95%CI=1.235–8.556; GC+CC and smoking: OR=2.167, 95%CI=1.175–3.996).

CONCLUSIONS: We found that rs3218408 was related with increased risk of CRC, and the interaction of rs3218384 with smoking increased the risk of CRC.

Keywords: Biodegradation, Environmental, Colorectal Neoplasms, Hereditary Nonpolyposis, Genes, vif