05 December 2017 : Laboratory Research
Emodin Impairs Radioresistance of Human Osteosarcoma Cells by Suppressing Sonic Hedgehog Signaling
Wei Qu12ADE, Yufei Wang3BDE, Qining Wu2ADF, Dingjun Hao2CDG*, Dichen Li45ADGDOI: 10.12659/MSM.907453
Med Sci Monit 2017; 23:5767-5773
Abstract
BACKGROUND: This study aimed to investigate the possible involvement of sonic hedgehog (Shh) signaling in the radioresistance of human osteosarcoma cells and the inhibitory effects of emodin on radioresistance.
MATERIAL AND METHODS: Human osteosarcoma (OS) cell line MG63 was employed as parent cells. After 30-repeat low-dose (2 Gy) x-ray irradiation on MG63 cells, radioresistant OS cell MG63R cells were produced. Colony formation assay was used to assess the radioresistance. Cell viability was evaluated by CCK-8 assay. TUNEL assay was used to detect cell apoptosis. Protein expression levels and nuclear translocation were evaluated by western blotting.
RESULTS: More survival colony formation, elevated cell viability, and less cell apoptosis were found in MG63R cells compared to MG63 cells exposed to irradiation. The nuclear translocation of glioma-associated oncogene homolog (Gli), expression levels of Shh and Bcl2 were increased in MG63R cells compared to MG63 cells. Emodin pretreatment significantly inhibited cell viability and survival colony formation but increased cell apoptosis of irradiation exposed MG63R cells in a concentration dependent manner. Moreover, emodin pretreatment inhibited Shh and Bcl2 expressions as well as Gli1 nuclear translocation but increased C-caspase-3 expression of irradiation exposed MG63R cells in a concentration dependent manner.
CONCLUSIONS: Shh signaling activation was involved in the radioresistance of human OS cells. Emodin impaired the radioresistant capacity of OS cells by inhibiting Shh signaling pathway.
Keywords: Emodin, Radiotherapy, Adjuvant
Editorial
01 January 2023 : Editorial
Editorial: Current Status of Two Adjuvanted Malaria Vaccines and the World Health Organization (WHO) Strategy to Eradicate Malaria by 2030DOI: 10.12659/MSM.939357
Med Sci Monit 2023; 29:e939357
SARS-CoV-2/COVID-19
26 January 2023 : Editorial
Editorial: The XBB.1.5 (‘Kraken’) Subvariant of Omicron SARS-CoV-2 and its Rapid Global SpreadMed Sci Monit In Press; DOI: 10.12659/MSM.939580
19 January 2023 : Clinical Research
Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...Med Sci Monit In Press; DOI: 10.12659/MSM.938892
19 January 2023 : Clinical Research
Determining the Impact of the COVID-19 Pandemic on Dental Care in the Serbian Adult Population: An Online Q...Med Sci Monit 2023; 29:e939238
27 December 2022 : Clinical Research
Effect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...Med Sci Monit 2022; 28:e938926
In Press
31 Jan 2023 : Clinical Research
Balloon Dilation Eustachian Tuboplasty Combined with Tympanotomy Tube Insertion for Treatment of Refractory...Med Sci Monit In Press; DOI: 10.12659/MSM.938879
31 Jan 2023 : Clinical Research
Comparison of Vision Correction and Corneal Thickness at 180-Day Follow-Up After Femtosecond Laser-Assisted...Med Sci Monit In Press; DOI: 10.12659/MSM.939099
30 Jan 2023 : Clinical Research
Splenic Artery Steal Syndrome in Patients with Liver Cirrhosis: A Retrospective Clinical StudyMed Sci Monit In Press; DOI: 10.12659/MSM.938998
27 Jan 2023 : Database Analysis
Association Between Neutrophil-Lymphocyte Ratio and All-Cause Mortality in Critically Ill Patients with Chr...Med Sci Monit In Press; DOI: 10.12659/MSM.938554
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952