06 December 2017 : Laboratory Research
Med Sci Monit 2017; 23:5783-5792
BACKGROUND: The purpose of this study was to explore changes in cartilage matrix in early-stage femoral head necrosis (FHN).
MATERIAL AND METHODS: Femoral head samples of patients with early FHN were collected during total hip arthroplasty (THA), high-field 7.0T MRI scans were performed in vitro, and the average T2 values were calculated. Cartilage samples were obtained from the weight-bearing area (FHN group) and non-weight-bearing area (Control group), divided into 3 equal parts and used for biochemical analysis, histopathological staining, and gene expression analysis.
RESULTS: T2 mapping of the femoral head specimens showed that the density distribution of cartilage surface was not uniform, and the average T2 value increased unevenly. Histological staining demonstrated that the number of chondrocytes was significantly decreased and they were irregularly arranged, SO staining was lost, and collagen fiber arrangement was slightly more irregular on the cartilage surface in the FHN group. The biochemical results in the FHN group showed that the water content increased significantly and the DNA content decreased significantly, while no significant changes in GAG and total collagen contents were detected. Gene expression analysis in the FHN group showed that SOX9 expression was significantly down-regulated, while COL10A1 and RUNX2 expressions were significantly up-regulated. The expression of ACAN and COL2A1 were decreased and COL1A1 was increased, but there was no significant difference compared with the Control group.
CONCLUSIONS: Taken together, the results of this study suggest that patients with early-stage FHN tend to have cartilage matrix degeneration, which provides new ideas for studying the pathogenesis of FHN and selecting treatment strategies.
Keywords: Femur Head Necrosis, Macular Degeneration, Magnetic Resonance Imaging, Matrilin Proteins
01 February 2024 : EditorialEditorial: Global Health Concerns as Vaccine-Preventable Infections Including SARS-CoV-2 (JN.1), Influenza, Respiratory Syncytial Virus (RSV), and Measles Continue to Rise
Med Sci Monit 2024; 30:e943911
05 Jan 2024 : Clinical ResearchEffectiveness of Glutamine Oral Care in Reducing Oral Mucositis and Improving Oral Health After Neurosurger...
Med Sci Monit In Press; DOI: 10.12659/MSM.942585
02 Jan 2024 : Clinical ResearchUrinary Concentration of Renal Biomarkers in Healthy Term Neonates: Gender Differences in GST-pi Excretion
Med Sci Monit In Press; DOI: 10.12659/MSM.942819
29 Dec 2023 : Clinical ResearchPerformance of Subcutaneous Implantable Cardioverter Defibrillator (S-ICD) in Chinese Population with Prima...
Med Sci Monit In Press; DOI: 10.12659/MSM.942747
Most Viewed Current Articles
16 May 2023 : Clinical ResearchElectrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...
Med Sci Monit 2023; 29:e940387
17 Jan 2024 : Review articleVaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical ResearchPrevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels
Med Sci Monit 2022; 28:e937990