11 September 2018 : Laboratory Research
Rs12976445 Polymorphism Is Associated with Post-Ablation Recurrence of Atrial Fibrillation by Modulating the Expression of MicroRNA-125a and Interleukin-6R
Xue-Bin Shen1ABDEFG, Shao-Hong Zhang2BCDF, Hai-Yang Li1DE, Xi-Di Chi2ABF, Ling Jiang1CDEF, Qi-Lei Huang1AEG*, Shang-Hua Xu1ACDGDOI: 10.12659/MSM.908555
Med Sci Monit 2018; 24: LBR6349-6358
Abstract
BACKGROUND: This study aimed to identify the relationship between miR-125a polymorphism rs12976445 and the post-ablation recurrence of atrial fibrillation (AF), as well as to explore the underlying mechanism of miR-125a in AF recurrence.
MATERIAL AND METHODS: Microarray analysis was performed to search for miRNAs potentially involved in the regulation of AF recurrence, while real-time PCR (polymerase chain reaction) and Western blot analyses were carried out to study the expression of miR-125a (microRNA-125a), IL-6R (interleukin-6 receptor), and IL-16 (interleukin-16) in different experimental groups, so as to understand the regulatory relationships among miR-125a, IL-6R, and IL-16. Subsequently, a logistic regression analysis was utilized to investigate the survival status of recurrent AF in subjects harboring different genotypes of rs12976445.
RESULTS: The subjects in the GG and GC/CC groups of miR-125a polymorphism rs12976445 showed no obvious difference regarding all demographic characteristics that were collected in this study. In addition, 19 miRNAs were identified as potentially involved in the regulation of AF recurrence. Among these miRNAs, 6 were upregulated and 13 were downregulated in the group with early recurrence. According to real-time PCR results, the expression of miR-125a was dramatically upregulated in LRAF (late recurrence of atrial fibrillation) as well as in subjects harboring the GG genotype. On the contrary, the level of IL-6R mRNA was dramatically downregulated in LRAF and subjects harboring the GG genotype. Furthermore, IL-6R was confirmed as a candidate target of miR-125a by a luciferase reporter assay.
CONCLUSIONS: MicroRNA-125a polymorphism rs12976445 plays a role in AF recurrence via the regulation of IL-6R.
Keywords: Atrial Fibrillation, Interleukin-6, Receptors, Interleukin-6
Editorial
01 February 2025 : Editorial
Editorial: Current Approaches to Screening for Lung Cancer in Smokers and Non-SmokersDOI: 10.12659/MSM.948255
Med Sci Monit 2025; 31:e948255
In Press
Clinical Research
Impact of Osteopathic Techniques on Autonomic Regulation: A Study of Heart Rate Variability in Healthy AdultsMed Sci Monit In Press; DOI: 10.12659/MSM.946903
Clinical Research
Immune Dysregulation in Acute Herpes Zoster: Predictive Factors for Postherpetic NeuralgiaMed Sci Monit In Press; DOI: 10.12659/MSM.944688
Clinical Research
Inflammatory Biomarkers from Blood Counts as Prognostic Tools in Metastatic Esophageal CancerMed Sci Monit In Press; DOI: 10.12659/MSM.947202
Laboratory Research
Comparison of Color Stability Between Single-Shade and Conventional Composite Resins Following Immersion in...Med Sci Monit In Press; DOI: 10.12659/MSM.946784
Most Viewed Current Articles
17 Jan 2024 : Review article 6,966,697
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research 701,689
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Mar 2024 : Editorial 25,052
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...DOI :10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
28 Jan 2024 : Review article 19,692
A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and FutureDOI :10.12659/MSM.943912
Med Sci Monit 2024; 30:e943912