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17 June 2018 : Laboratory Research  

Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis

Ying Zhang1BE, Qian Xin2C, Zhen Wu1D, Chaochao Wang1D, Yongbin Wang1B, Qian Wu1F, Rui Niu1AG*

DOI: 10.12659/MSM.908702

Med Sci Monit 2018; 24: LBR4146-4153

Abstract

BACKGROUND: The present study was performed to explore the presence of informative protein biomarkers of human serum proteome in idiopathic pulmonary fibrosis (IPF).

MATERIAL AND METHODS: Serum samples were profiled using iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) technique, and ELISA was used to validate candidate biomarkers.

RESULTS: A total of 394 proteins were identified and 97 proteins were associated with IPF. Four biomarker candidates generated from iTRAQ experiments – CRP, fibrinogen-α chain, haptoglobin, and kininogen-1 – were successfully verified using ELISA.

CONCLUSIONS: The present study demonstrates that levels of CRP and fibrinogen-a are higher and levels of haptoglobin and kininogen-1 are lower in patients with IPF compared to levels in healthy controls. We found they are useful candidate biomarkers for IPF.

Keywords: Biological Markers, idiopathic pulmonary fibrosis, Proteomics

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750