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22 February 2018 : Animal Research  

Mineralization Induction of Gingival Fibroblasts and Construction of a Sandwich Tissue-Engineered Complex for Repairing Periodontal Defects

Mingxuan Wu1BCDEG, Jie Wang2AEFG*, Yanning Zhang2BDE, Huijuan Liu2D, Fusheng Dong3ACF

DOI: 10.12659/MSM.908791

Med Sci Monit 2018; 24: ANS1112-1123

Abstract

BACKGROUND: The ideal healing technique for periodontal tissue defects would involve the functional regeneration of the alveolar bone, cementum, and periodontal ligament, with new periodontal attachment formation. In this study, gingival fibroblasts were induced and a “sandwich” tissue-engineered complex (a tissue-engineered periodontal membrane between 2 tissue-engineered mineralized membranes) was constructed to repair periodontal defects. We evaluated the effects of gingival fibroblasts used as seed cells on the repair of periodontal defects and periodontal regeneration.

MATERIAL AND METHODS: Primitively cultured gingival fibroblasts were seeded bilaterally on Bio-Gide collagen membrane (a tissue-engineered periodontal membrane) or unilaterally on small intestinal submucosa segments, and their mineralization was induced. A tissue-engineered sandwich was constructed, comprising the tissue-engineered periodontal membrane flanked by 2 mineralized membranes. Periodontal defects in premolar regions of Beagles were repaired using the tissue-engineered sandwich or periodontal membranes. Periodontal reconstruction was compared to normal and trauma controls 10 or 20 days postoperatively.

RESULTS: Periodontal defects were completely repaired by the sandwich tissue-engineered complex, with intact new alveolar bone and cementum, and a new periodontal ligament, 10 days postoperatively.

CONCLUSIONS: The sandwich tissue-engineered complex can achieve ideal periodontal reconstruction rapidly.

Keywords: Fibroblasts, Gingiva, mandibular reconstruction, periodontal diseases, Tissue Engineering

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750