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16 August 2018 : Laboratory Research  

Circular RNA Complement Factor H (CFH) Promotes Glioma Progression by Sponging miR-149 and Regulating AKT1

Aimiao Bian1ABCDEF, Yanping Wang1ABCDEF, Ji Liu1D, Xiaodong Wang1F, Dai Liu1C, Jian Jiang1CD, Lianshu Ding1CE, Xiaobo Hui1ABCEG*

DOI: 10.12659/MSM.910180

Med Sci Monit 2018; 24: LBR5704-5712

Abstract

BACKGROUND: Circular RNAs (circRNAs) are widely expressed in mammals and can regulate the development and progression of human tumors. has_circ_0015758 (circ-CFH) is an exon circRNA transcript from the GRCh37/hg19 fragment of chromosome 1 and is homologous to the protein-coding gene complement factor H (CFH). Currently, the function of circ-CFH in glioma remains unclear.

MATERIAL AND METHODS: In our study, circ-CFH, miR-149, and Akt1 mRNA expression levels were analyzed by qRT-PCR assays. To investigate the function of circ-CFH in cell proliferation, circ-CFH knockdown models were established by using circ-CFH siRNAs. Cell proliferation abilities were measured by CCK-8 and colony formation assays and in vivo experiments. In addition, the interaction between circ-CFH and miR-149 was assessed by luciferase reporter assays.

RESULTS: Circ-CFH expression was significantly upregulated in glioma tissue and was correlated with tumor grade. Circ-CFH expression levels were also markedly higher in U251 and U373 glioma cell lines. Circ-CFH knockdown inhibited cell proliferation and colony formation abilities. Luciferase assays indicated that circ-CFH functions as a miR-149 sponge and inhibits its function in U251 and U373 cells. Subsequently, AKT1 was identified as a direct target of the circ-CFH/miR-149 axis.

CONCLUSIONS: Circ-CFH promotes glioma progression by sponging miR-149 and regulating the AKT1 signaling pathway. The circ-CFH/miR-149/AKT1 regulation axis may be a potential target for glioma therapy.

Keywords: Astrocytoma, RNA, Small Untranslated

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750