05 September 2018 : Animal Research
Modulation of P2X Purinoceptor 3 (P2X3) in Pentylenetetrazole-Induced Kindling Epilepsy in Rats
Jie Xia1ABCDEFG*, Hui Wang2BCF, Qimei Zhang1ABFG, Zhongmou Han1BDEFDOI: 10.12659/MSM.910352
Med Sci Monit 2018; 24: ANS6165-6177
Abstract
BACKGROUND: Epilepsy is a complex neurologic disorder with abnormal electrical impulses in the brain. A crucial role of purinergic signalling in the proper working of the nervous system has been reported but much less is known about the modulation of P2X3 purinergic receptors in epilepsy. This study investigated the effect of NF110, a potent P2X3 receptor antagonist, in the rat epilepsy model of pentylenetetrazole (PTZ)-induced kindling.
MATERIAL AND METHODS: The mean kindling score, motor activity, locomotion, emotional tension, anxiety, discrimination ability, learning, memory, serum neuron-specific enolase (sNSE), hippocampal IL-1β and TNF-α, thiobarbituric acid-reactive substance (TBARS), catalase (CAT) and reduced glutathione (GSH), and mitochondrial complex I, II, and IV levels of PTZ-kindling animals were assessed.
RESULTS: The PTZ-kindling animals have shown impaired motor activity, locomotion, discrimination ability, learning, and memory, along with increased emotional tension, anxiety, neuronal damage (increased sNSE), hippocampal pro-inflammatory mediators (increased IL-1β and TNF-α), oxidative stress (increased TBARS, decreased GSH and CAT), and mitochondrial dysfunction. The administration of NF110 in 3 different doses has significantly and dose-dependently corrected PTZ-kindling-induced impaired behavior, learning, memory, locomotion, motor activity, discrimination ability, neuronal damage, hippocampal inflammation, oxidative stress, and mitochondrial dysfunction. These beneficial effects of NF110 in PTZ-kindling animals were significantly abolished by the administration of the P2X agonist α, β methylene-ATP.
CONCLUSIONS: P2X3 receptors play a very important role in kindling epilepsy and further research should be done to design P2X3 modulators for their possible therapeutic benefits in epileptic disorders.
Keywords: Hippocampus, Receptors, Purinergic P2X
Editorial
01 February 2025 : Editorial
Editorial: Current Approaches to Screening for Lung Cancer in Smokers and Non-SmokersDOI: 10.12659/MSM.948255
Med Sci Monit 2025; 31:e948255
In Press
Clinical Research
Impact of Osteopathic Techniques on Autonomic Regulation: A Study of Heart Rate Variability in Healthy AdultsMed Sci Monit In Press; DOI: 10.12659/MSM.946903
Clinical Research
Immune Dysregulation in Acute Herpes Zoster: Predictive Factors for Postherpetic NeuralgiaMed Sci Monit In Press; DOI: 10.12659/MSM.944688
Clinical Research
Inflammatory Biomarkers from Blood Counts as Prognostic Tools in Metastatic Esophageal CancerMed Sci Monit In Press; DOI: 10.12659/MSM.947202
Laboratory Research
Comparison of Color Stability Between Single-Shade and Conventional Composite Resins Following Immersion in...Med Sci Monit In Press; DOI: 10.12659/MSM.946784
Most Viewed Current Articles
17 Jan 2024 : Review article 6,966,697
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research 701,689
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Mar 2024 : Editorial 25,052
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and ...DOI :10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
28 Jan 2024 : Review article 19,692
A Review of IgA Vasculitis (Henoch-Schönlein Purpura) Past, Present, and FutureDOI :10.12659/MSM.943912
Med Sci Monit 2024; 30:e943912