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05 June 2018 : Animal Research  

Effect of Xylosyltransferase-I Silencing on Implanting Growth of Salivary Pleomorphic Adenoma

Guiyun Ren1ABCDE, Yanning Zhang2BCEF, Jie Wang2ABCEG*, Huijuan Liu2B, Fusheng Dong1ADF

DOI: 10.12659/MSM.911014

Med Sci Monit 2018; 24: ANS3772-3781


BACKGROUND: Salivary pleomorphic adenoma is one of the most common salivary gland tumors. It has a relatively high tendency to recur and a high risk of malignant transformation. The present study aimed to study the effect of XT-I gene silencing on the implanting growth of salivary pleomorphic adenoma.

MATERIAL AND METHODS: Primary cultures of SPA cells and fibroblasts from the same patient were assessed. The adenovirus vector Ad-shRNA-XT-I was constructed and transfected into SPA cells. The expression of XT-I gene and XT-I protein was detected by real-time PCR and Western blot. The contents of proteoglycans were detected. The SPA cells transfected with Ad-shRNA-XT-I (group SPA-XT-I) and Ad-shRNA-HK (group SPA-HK), as well as without transfection (group SPA), were implanted into ADM scaffold with fibroblasts and then transferred into 18 BALB/C-nu nude mice for 3 months.

RESULTS: Primary cultures showed SPA cells were positive for human CK and S-100 protein and the fibroblasts were positive for human vimentin. The expressions of XT-I gene and protein were decreased by 51% and 51.31%, respectively. The content of proteoglycans was reduced by 48.45%. The results of the implanting growth in vitro and in vivo of nude mice indicated that no tumors grew in the SPA-XT-I group, whereas SPA grew in groups SPA-HK and SPA positive for human a-SMA, S-100 protein, and calponin.

CONCLUSIONS: XT-I gene silencing effectively inhibited the implanting growth of SPA.

Keywords: Adenoma, Pleomorphic, Proteoglycans, RNA Interference, Tissue Engineering

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750