Abstract

BACKGROUND: The transcription factor Oct-4 is necessary for maintaining pluripotency and self-renewal of embryonic stem cells, and POU5F1B is a processed pseudogene of Oct-4 with coding capacity. The purpose of this study is to evaluate the expression and clinical implication of POU5F1B in AML.

MATERIAL AND METHODS: The expression of the POU5F1B transcript was evaluated in 175 newly diagnosed AML patients and 39 healthy controls by use of real-time quantitative PCR (RQ-PCR).

RESULTS: POU5F1B was underexpressed in AML compared with controls (P<0.001). The receiver operating characteristic (ROC) curve revealed that the POU5F1B transcript level was able to differentiate AML patients from healthy individuals (AUC=0.682). In non-APL AML patients, the POU5F1Blow group had significantly higher WBC than the POU5F1Bhigh group (20.2×10⁹ vs. 4.6×10⁹ L⁻¹, P=0.021). Among whole-cohort AML, non-APL AML, and intermediate-risk AML, POU5F1Bhigh patients had obviously higher complete remission (CR) rates than POU5F1Blow patients (P=0.012, P=0.012 and P=0.027). In addition, Kaplan-Meier analysis demonstrated better overall survival (OS, P=0.019, P=0.007 and P=0.046, respectively) in POU5F1Bhigh patients compared with POU5F1Blow patients. Furthermore, in multivariate survival analysis, POU5F1B was independently associated with OS in non-APL AML patients and intermediate-risk AML as a favorable prognostic factor.

CONCLUSIONS: POU5F1B was frequently underexpressed in AML, and might contribute to the diagnosis and prognosis of AML.

Keywords: Biological Markers, Diagnosis, Leukemia, Myeloid, Acute, Prognosis, Pseudogenes, Aged, Aged, 80 and over, Area Under Curve, Biomarkers, Tumor, Genes, Homeobox, Kaplan-Meier Estimate, Middle Aged, Octamer Transcription Factor-3, ROC Curve, Remission Induction, transcriptome