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28 June 2019 : Laboratory Research  

Effects and Mechanism of Tanshinone II A in Proliferation, Apoptosis, and Migration of Human Colon Cancer Cells

Junlai Xue1BCD, Xuezhu Jin2CDE, Xuewen Wan1DEF, Xiangdang Yin3EFG, Meishan Fang1DEF, Tongjun Liu4ABEF*, Shuhua Zhao1ABCE

DOI: 10.12659/MSM.914446

Med Sci Monit 2019; 25:4793-4800


BACKGROUND: The aim of this study was to explore the effect and mechanism of tanshinone II A on proliferation, apoptosis, and migration of human colon cancer cells.

MATERIAL AND METHODS: CCK-8 approach was carried out to evaluate proliferation after applying various levels of tanshinone II A to SW620 colon carcinoma cells. Flow cytometry (FC) was used to assess apoptosis. Transwell assay was performed to assess invasion in vitro, and the wound-healing assay was applied to assess migration. Western blot analysis was performed to evaluate translation of mTOR, while RT-PCR was carried out to assess transcription of VEGF.

RESULTS: CCK-8 assay showed that tanshinone II A inhibited SW620 proliferation in comparison to the control group subsequent to 24 h, 48 h, and 72 h (P<0.001). FC revealed that tanshinone II A promoted SW620 apoptosis (P<0.001). The cell migration test revealed that the migration index of cells receiving tanshinone II A decreased. mTOR translation as well as VEGE transcription in cells receiving tanshinone II A was noticeably prohibited compared to control group (P<0.001).

CONCLUSIONS: Tanshinone II A is able to inhibit proliferation and migration of human colon cancer SW620 cells and promoted cell death. Its mechanism may be by downregulation of mTOR protein and VEGF mRNA.

Keywords: Arginase, Lynch Syndrome II, Sigmoid Neoplasms, Abietanes, Antineoplastic Agents, Phytogenic, Colonic Neoplasms, Down-Regulation

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750