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23 July 2019 : Animal Research  

Treatment Effect of Tuftsin and Antigen Peptide Combined with Immune Cells on Colorectal Cancer

Boyuan Zou12ABCEF, Shaoyou Xia2AC, Xiaohui Du2AE*, Yingxin Xu2A, Ning Ning23C, Songyan Li2DF, Da Teng2CDF, Hao Li2CF, Zilong Hu2D, Shidong Hu2CE, Yufeng Wang2F

DOI: 10.12659/MSM.915037

Med Sci Monit 2019; 25:5465-5472


BACKGROUND: The aim of this study was to investigate the effect of antigenic peptides on dendritic cell maturation and activation as well as the role of dendritic cell induced cell function. The tumor-specific cytotoxic T lymphocytes induced by activation of the dendritic cells were also evaluated.

MATERIAL AND METHODS: SW-480 cell lysate and peptide antigens were selected as adjuvants in dendritic cell sensitization, and tuftsin was used to induce the phagocytosis of dendritic cells. Immature dendritic cells were stimulated with the antigen and adjuvant as follows: group A was negative control; group B was SW-480 (20 μg/mL); group C was SW-480 (20 μg/mL)+tumor necrosis factor (TNF)-α (10 μg/mL); group D was SW-480 (20 μg/mL)+tuftsin (20 μg/mL); group E was antigen peptide (2 μg/mL); group F was antigen peptide (2 μg/mL)+TNF-α (10 μg/mL); group G was antigen peptide (2 μg/mL)+tuftsin (20 μg/mL). Cytotoxic T lymphocytes activation and in vitro anti-tumor effects were examined by detecting the maturation marks of dendritic cells as well as interleukin (IL)-10 and IL-12 levels secreted by dendritic cells. Cells with the strongest immunizing effects were injected into nude mice and tumor suppression status was evaluated.

RESULTS: Group D (SW-480+tuftsin), group E (antigen peptides), group F (antigen peptide+TNF-α), and group G (antigen peptides+tuftsin) displayed significant differences compared to the control group (P<0.05). Group G (antigen peptides+tuftsin) could also promote the secretion of cytokines IL-12, as well as inhibit cytokine IL-10 secretion, compared to the other experimental groups (P<0.05). In the in vivo experiments of tumor inhibitions, antigenic polypeptide+tuftsin was the most effective (P<0.05).

CONCLUSIONS: Combination of cytotoxic T lymphocytes and T peptide therapy in treating human colorectal cancer might be used as a new treatment strategy based on adoptive cellular immunotherapy.

Keywords: Colorectal Neoplasms, Immunotherapy, Adoptive, Tuftsin, Colonic Neoplasms, Lymphocyte Activation, Peptides, T-Lymphocytes, Cytotoxic



01 March 2023 : Editorial  

Editorial: Global Surveillance of Highly Pathogenic Avian Influenza Viruses in Poultry, Wild Birds, and Mammals to Prevent a Human Influenza Pandemic

Dinah V. Parums

DOI: 10.12659/MSM.939968

Med Sci Monit 2023; 29:e939968


03 March 2023 : Database Analysis  

Reinfections from SARS-CoV-2: A Retrospective Study from the Gyncentrum Genetic Laboratory in Sosnowiec, Po...

Med Sci Monit In Press; DOI: 10.12659/MSM.939452  

09 February 2023 : Clinical Research  

Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...

Med Sci Monit 2023; 29:e938892

27 December 2022 : Clinical Research  

Effect of Physiotherapy to Correct Rounded Shoulder Posture in 30 Patients During the COVID-19 Pandemic in ...

Med Sci Monit 2022; 28:e938926

In Press

21 Mar 2023 : Clinical Research  

Efficacy of Direct Anterior Approach Versus Posterior Lateral Approach for Total Hip Replacement in Patient...

Med Sci Monit In Press; DOI: 10.12659/MSM.939682  

21 Mar 2023 : Clinical Research  

Intraoperative Hypotension and Its Association with Postoperative Acute Kidney Injury in Patients Undergoin...

Med Sci Monit In Press; DOI: 10.12659/MSM.938945  

20 Mar 2023 : Clinical Research  

Management of Unstable C1 Fractures Involving the Lateral Mass: Posterior C1-C2 Screw-Rod Fixation and Fusion

Med Sci Monit In Press; DOI: 10.12659/MSM.938600  

17 Mar 2023 : Clinical Research  

Relationship Between Autonomic Nervous System Activity and Axial Length in Children

Med Sci Monit In Press; DOI: 10.12659/MSM.939451  

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750