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17 August 2019 : Laboratory Research  

Role of CircRNAs_100395 in Proliferation and Metastases of Liver Cancer

Qiaming Chen1ABC, Zhian Chen2AEF, Sai Cao3DEF, Botang Guo2DEF, Yilin Chen2DEF, Zixin Feng2DE, Jiaqi Wang2DEF, Guohu Guo4ADF, Xinhua Chen2ABCG, Xianying Huang5CDE*

DOI: 10.12659/MSM.915963

Med Sci Monit 2019; 25:6181-6192

Abstract

BACKGROUND: Circular RNAs (circRNAs) are a kind of noncoding RNA with high cancer-specific expression, and great potential in regulating tumorigenesis. Among these, circRNA_100395 (circ_100395) has been reported to be downregulated in lung cancer, and participates in the process of tumor cell proliferation and metastasis. However, its expression and function in liver cancer remain unknown.

MATERIAL AND METHODS: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to evaluate the expression level of circ_100395 and microRNAs-1228 (miR-1228) in liver cancer samples and the adjacent non-tumor tissues. Cell proliferation, apoptosis, invasion, migration, and epithelial-mesenchymal transition (EMT) pathway of circ_100395 upregulated cells were analyzed using a Cell Counting Kit-8 (CCK-8), flow cytometry, Transwell assay, and Western blot analysis.

RESULTS: We found that circ_100395 was downregulated in cancerous liver tissues relative to the adjacent normal tissues. The overexpression of circ_100395 was negatively associated with tumor differentiation, microvascular invasion, and portal vein tumor thrombosis. However, patients with higher circ_10039 expression tended to have better postoperative disease-free survival time. Moreover, upregulation of circ_100395 in liver cancer cells inhibited cell proliferation, induced apoptosis, then silenced the EMT pathway and reduced migration and invasion abilities, while this anti-tumor effect was significantly reversed by the downstream target, miR-1228.

CONCLUSIONS: circ_100395 appears to be a promising therapeutic target for liver cancer.

Keywords: Carcinoma, Hepatocellular, Epithelial-mesenchymal transition, Neoplasm Metastasis, Aged, Aged, 80 and over, Apoptosis, carcinogenesis, Gene Expression Profiling, Liver Neoplasms, Middle Aged, Neoplasm Invasiveness, RNA, Circular, Real-Time Polymerase Chain Reaction

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750