10 October 2019 : Laboratory Research
The Effect of Ras Homolog C/Rho-Associated Coiled-Protein Kinase (Rho/ROCK) Signaling Pathways on Proliferation and Apoptosis of Human Myeloma Cells
Xianqi Feng1BDF, Ling Zhang2ACE, Shumin Nie3DF, Likun Zhuang4BE, Wei Wang1AD, Junxia Huang1CF, Xueshen Yan1BD, Fanjun Meng1AEG*DOI: 10.12659/MSM.915998
Med Sci Monit 2019; 25:7605-7616
Abstract
BACKGROUND: The aim of this study was to explore the impact of Ras homolog C/Rho-associated coiled-protein kinase (Rho/ROCK) signaling pathways intervention on biological characteristics of the human multiple myeloma cell lines RPMI-8226 and U266 cells, and to investigate the expression of RhoC, ROCK1, and ROCK2 in RPMI-8226 and U266 cells.
MATERIAL AND METHODS: RPMI8226 and U266 cell lines were treated by 5-aza-2-deoxycytidine (5-Aza-Dc), trichostatin A (TSA), RhoA inhibitor CCG-1423, Rac1 inhibitor NSC23766, and ROCK inhibitor fasudil. Cell proliferation was examined by Cell Counting Kit-8 (CCK-8) assay and clone formation. Cell apoptosis was examined by flow cytometry and TUNEL assay. The mRNA and protein expressions of RhoC, ROCK1, and ROCK2 were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot, respectively.
RESULTS: CCG-1423, NSC23766, and fasudil could significantly inhibit the proliferation of RPMI8226 and U266 cells. The inhibitory effect was dose- and time-dependent within a certain concentration range (P<0.05). After treatment with CCG-1423, NSC23766, and fasudil for 24 hours, the apoptosis rates of RPMI8226 and U266 cells were significantly higher than those of the control group, which were dose-dependent (P<0.05). Compared with the control group, the mRNA and protein expressions of RhoC, ROCK1, and ROCK2 in RPMI8226 and U266 cells were significantly decreased with single 5-Aza-Dc or TSA treatment. However, the effects were obviously stronger after combined treatment of 5-Aza-CdR and TSA (P<0.05).
CONCLUSIONS: We found that 5-Aza-Dc and TSA can effectively decrease the mRNA and protein expressions of RhoC, ROCK1, and ROCK2. Furthermore, Rho and ROCK inhibitors significantly inhibit cell growth and induce cell apoptosis in the human multiple myeloma cell lines RPMI-8226 and U266.
Keywords: Multiple Myeloma, Population Characteristics, rho-Associated Kinases, 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine, Azacitidine, Hydroxamic Acids, Protein Kinase Inhibitors, RNA, Messenger, rho GTP-Binding Proteins
Editorial
01 March 2023 : Editorial
Editorial: Global Surveillance of Highly Pathogenic Avian Influenza Viruses in Poultry, Wild Birds, and Mammals to Prevent a Human Influenza PandemicDOI: 10.12659/MSM.939968
Med Sci Monit 2023; 29:e939968
SARS-CoV-2/COVID-19
29 March 2023 : Editorial
Editorial: Effective Direct-Acting Antiviral Treatments Support Global and National Programs to Eliminate H...Med Sci Monit In Press; DOI: 10.12659/MSM.940519
30 March 2023 : Clinical Research
An Online Survey on the Relationship Between Positive Coping and Post-Traumatic Stress Symptoms (PTSS) of M...Med Sci Monit In Press; DOI: 10.12659/MSM.939485
09 February 2023 : Clinical Research
Evaluation of Health-Related Quality of Life and Mental Health in 729 Medical Students in Indonesia During ...Med Sci Monit 2023; 29:e938892
19 January 2023 : Clinical Research
Determining the Impact of the COVID-19 Pandemic on Dental Care in the Serbian Adult Population: An Online Q...Med Sci Monit 2023; 29:e939238
In Press
30 Mar 2023 : Clinical Research
An Online Survey on the Relationship Between Positive Coping and Post-Traumatic Stress Symptoms (PTSS) of M...Med Sci Monit In Press; DOI: 10.12659/MSM.939485
30 Mar 2023 : Database Analysis
Global Research Trends and Hotspots in Lateral Epicondylitis During the Past 30 Years: A Bibliometric and V...Med Sci Monit In Press; DOI: 10.12659/MSM.939309
29 Mar 2023 : Editorial
Editorial: Effective Direct-Acting Antiviral Treatments Support Global and National Programs to Eliminate H...Med Sci Monit In Press; DOI: 10.12659/MSM.940519
29 Mar 2023 : Laboratory Research
Preparation, Physicochemical Properties, and Structural Characterization of Resistant Starch-Aspirin-Loaded...Med Sci Monit In Press; DOI: 10.12659/MSM.939711
Most Viewed Current Articles
13 Nov 2021 : Clinical Research
Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...DOI :10.12659/MSM.932788
Med Sci Monit 2021; 27:e932788
30 Dec 2021 : Clinical Research
Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...DOI :10.12659/MSM.935379
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review article
A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...DOI :10.12659/MSM.936292
Med Sci Monit 2022; 28:e936292
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952